MAB3503-I

Anti-Apaf-1 Antibody, clone 2E12

clone 2E12, from rat

• 別名: Apoptotic protease-activating factor 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 2E12, monoclonal
• 交差性: human
• テクニック: electron microscopy: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_863651
• UniProtアクセッション番号: O14727
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... APAF1(317)

詳細

詳細

Apoptotic protease-activating factor 1 (UniProt: O14727; also known as Apaf-1) is encoded by the Apaf-1 gene (Gene ID: 317) in human. Apaf-1 contains a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. It is highly expressed in lung and spleen, weakly in brain and kidney and is not detectable in liver. Under basal conditions it is a monomeric protein, but oligomerizes under apoptotic conditions. The monomeric form is auto-inhibited in a closed conformation through a bound ADP at the nucleotide binding site. Under apoptosis signals ADP is exchanged for ATP and its binding to cytochrome c triggers a conformation change where WD repeat region swings out, which allows the NB-ARC domain to rotate and expose the contact areas for oligomerization. Oligomeric form is a heptameric ring that binds to cytochrome c and ATP and is known as the apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 to mature caspase-9, which then activates effector caspases 3, 6, and 7.

特異性

Clone 2E12 detects Apaf-1 in Jurkat cells. It targets an epitope within first 97 amino acids from the N-terminal region.

免疫原

Epitope: N-terminus

Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

アプリケーション

Research Category
アポトーシス及び癌

This rat monoclonal Anti-Apaf-1 Antibody, clone 2E12, Cat. No. MAB3503-I, has been tested for use in Electron Microscopy, Immunocytochemistry, Immunoprecipitation, and Western Blotting for the detection Apaf-1.

Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Hausmann, G., et. al. (2000). J Cell Biol. 149(3):623-34).

Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Moriishi, K., et. al. (1999). Proc Natl Acad Sci USA. 96(17):9683-8).

Immunocytochemistry Analysis: A representative lot detected Apaf-1 in Immunocytochemistry applications (Hausmann, G., et. al. (2000). J Cell Biol. 149(3):623-34).

Immunoprecipitation Analysis: A representative lot detected Apaf-1 in Immunoprecipitation applications (Moriishi, K., et. al. (1999). Proc Natl Acad Sci USA. 96(17):9683-8).

Electron Microscopy Analysis: A representative lot detected Apaf-1 in Electron Microscopy applications (Hausmann, G., et. al. (2000). J Cell Biol. 149(3):623-34).

品質

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Apaf-1 in 10 µg of Jurkat cell lysate.

ターゲットの説明

~140 kDa observed; 141.84 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2a in PBS with 0.02% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2