おすすめの製品
由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
1.D5, monoclonal
化学種の反応性
human, rat
メーカー／製品名
Chemicon®
テクニック
western blot: suitable
アイソタイプ
IgG1
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... APP(351)
特異性
Reacts with the natural amyloid precursor protein A4(695).
免疫原
Epitope: A4(695)
Synthetic peptide Alz 90 [amino acids 511-608 of amyloid precursor protein pre A4(695)].
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経変性疾患
神経変性疾患
This Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5 is validated for use in WB for the detection of Amyloid Precursor Protein.
Western blot: 10-20 μg/mL
Optimal working dilutions must be determined by the end user.
Westen Blot Protocol for MAB349
1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.
2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.
3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.
4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.
5) Repeat step #3.
6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.
7) Repeat step #3.
8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.
9) Repeat step #3.
Optimal working dilutions must be determined by the end user.
Westen Blot Protocol for MAB349
1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.
2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.
3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.
4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.
5) Repeat step #3.
6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.
7) Repeat step #3.
8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.
9) Repeat step #3.
物理的形状
Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
保管および安定性
Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB349:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Roles of focal adhesions and fibronectin-mediated cohesion in proliferation of confluent fibroblasts.
Maya Tanaka,Tatsuya Abe,Yoshitaka Hara
Journal of Cellular Physiology null
Axonal injury in young pediatric head trauma: a comparison study of ?-amyloid precursor protein (?-APP) immunohistochemical staining in traumatic and nontraumatic deaths.
Johnson, MW; Stoll, L; Rubio, A; Troncoso, J; Pletnikova, O; Fowler, DR; Li, L
Journal of Forensic Sciences null
Magdolna Pákáski et al.
Neurochemistry international, 47(3), 190-195 (2005-06-16)
Comorbid depression of Alzheimer's disease (AD) is a common mood disorder in the elderly and a broad spectrum of antidepressants have been used for its treatment. Abeta peptides and other derivatives of the amyloid precursor protein (APP) have been implicated
Identification, biogenesis, and localization of precursors of Alzheimer's disease A4 amyloid protein.
Weidemann, A, et al.
Cell, 57, 115-126 (1989)
3,4-Methylenedioxymethamphetamine (MDMA), but not morphine, alters APP processing in the rat brain.
Kalman, Janos, et al.
The International Journal of Neuropsychopharmacology, 10, 183-190 (2007)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）