由来生物
mouse
抗体製品の状態
ascites fluid
クローン
SP14, monoclonal
化学種の反応性
pig, human, hamster, rat
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
アイソタイプ
IgG1
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... SNAP25(6616)
特異性
SNAP-25 (26-27 kDa). MAB331 is reactive with fusion protein from COS cells, but not with fusion protein from bacterial systems.
免疫原
Crude synaptic immunoprecipitate (Human)
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
シナプス及びシナプス生物学
シナプス及びシナプス生物学
Detect SNAP-25 using this Anti-SNAP-25 Antibody, clone SP14 validated for use in ELISA, WB, IH(P).
Immunohistochemistry at 1:500-1:5,000
Formalin fixed (<4 weeks), paraffin embedded
* It is suggested that you use an IgG specific second antibody to minimize possible non-specific staining.
Frozen
Western blotting at 1:100-1:1,000
ELISA at 1:500
It is reported that clone SP14 is capable of detecting SDS-resistant SNARE complexes {J. Cell Sci 2004 117(6):955-966}.
Optimal working dilutions must be determined by the end user.
Formalin fixed (<4 weeks), paraffin embedded
* It is suggested that you use an IgG specific second antibody to minimize possible non-specific staining.
Frozen
Western blotting at 1:100-1:1,000
ELISA at 1:500
It is reported that clone SP14 is capable of detecting SDS-resistant SNARE complexes {J. Cell Sci 2004 117(6):955-966}.
Optimal working dilutions must be determined by the end user.
物理的形状
Liquid. Contains no preservative.
保管および安定性
Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB331:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Bronchoepithelial expression of CXCR1 and CXCR2 does not facilitate transepithelial migration of neutrophils.
Christian Schulz,Benjamin Stoelcker,Brigitte Ruhland,Nico Jentsch,Andreas Steege
Respiration; International Review of Thoracic Diseases null
Matthew J Van Hook et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(17), 4618-4634 (2017-04-02)
G-protein βγ subunits (Gβγ) interact with presynaptic proteins and regulate neurotransmitter release downstream of Ca2+ influx. To accomplish their roles in sensory signaling, photoreceptor synapses use specialized presynaptic proteins that support neurotransmission at active zone structures known as ribbons. While
High sensitivity of mouse neuronal cells to tetanus toxin requires a GPI-anchored protein.
Munro, P; Kojima, H; Dupont, JL; Bossu, JL; Poulain, B; Boquet, P
Biochemical and biophysical research communications null
The gene for soluble N-ethylmaleimide sensitive factor attachment protein alpha is mutated in hydrocephaly with hop gait (hyh) mice.
Hong, HK; Chakravarti, A; Takahashi, JS
Proceedings of the National Academy of Sciences of the USA null
Most peptide-containing sensory neurons lack proteins for exocytotic release and vesicular transport of glutamate.
Morris, JL; Konig, P; Shimizu, T; Jobling, P; Gibbins, IL
The Journal of Comparative Neurology null
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