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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
MB3.6, monoclonal
化学種の反応性
human
メーカー／製品名
Chemicon®
テクニック
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
アイソタイプ
IgG3
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
関連するカテゴリー
特異性
Reacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells.
免疫原
FM9 melanoma cells
アプリケーション
Anti-Disialoganglioside GD3 Antibody, clone MB3.6 is an antibody against Disialoganglioside GD3 for use in FC, IF, IH.
Immunohistochemistry (frozen tissue sections)
Immunofluorescence of cells in culture
Flow cytometry: 1-2 μg per 10E6 cells
Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).
Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.
TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}
Optimal working dilutions must be determined by end user.
Immunofluorescence of cells in culture
Flow cytometry: 1-2 μg per 10E6 cells
Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).
Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.
TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}
Optimal working dilutions must be determined by end user.
物理的形状
Format: Purified
Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% NaN3.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Characterization of new serum biomarkers in breast cancer using lipid microarrays.
Yoshiya Yonekubo,Ping Wu,Aimalohi Esechie,Yueqiang Zhang,Guangwei Du
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine null
Analysis of effector cells in human antibody-dependent cellular cytotoxicity with murine monoclonal antibodies.
Ortaldo, JR et al.
Journal of immunology (Baltimore, Md. : 1950), 138(10), 3566-3572 (1987)
Biosynthesis and expression of the disialoganglioside GD2, a relevant target antigen on small cell lung carcinoma for monoclonal antibody-mediated cytolysis.
Cheresh, D A, et al.
Cancer Research, 46, 5112-5118 (1986)
Suzanne M Birks et al.
Neuro-oncology, 13(9), 950-960 (2011-08-03)
The expression of ganglioside GD3, which plays crucial roles in normal brain development, decreases in adults but is upregulated in neoplastic cells, where it regulates tumor invasion and survival. Normally a buildup of GD3 induces apoptosis, but this does not
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