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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
DB7, monoclonal
化学種の反応性
human, rabbit, guinea pig
以下との反応禁止
rat, mouse
メーカー／製品名
Chemicon®
テクニック
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
アイソタイプ
IgG2a
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... TNC(3371)
特異性
The antibody recognizes the Mr 250000 and 180000 tenascin polypeptides in immunoprecipitation and immunoblots the Mr 250000 polypeptide. The antibody reacts with the fibrinogen-like knob-domain of tenascin protein
免疫原
Tenascin polypeptides isolated from spent culture supernatant of human fibroblasts isolated by affinity chromatography.
アプリケーション
Research Category
細胞骨格
細胞骨格
Research Sub Category
ECMタンパク質
ECMタンパク質
Detect Tenascin using this Anti-Tenascin Antibody, clone DB7 validated for use in IF, WB, IH(P).
Immunofluorescence: 1-2% PFA fixed, permeabilized tissues or cell cultures. Mild pepsin digestion improves staining.
Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.
Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.
Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)
Final dilutions must be determined by end user.
Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.
Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.
Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)
Final dilutions must be determined by end user.
関連事項
Replaces: MAB1902
物理的形状
Format: Purified
The purified antibody is in PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.
保管および安定性
Maintain refrigerated at 2-8°C
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB19101:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Tenascin immunoreactivity as a prognostic marker in usual interstitial pneumonia.
Kaarteenaho-Wiik, R, et al.
American Journal of Respiratory and Critical Care Medicine, 154, 511-518 (1996)
Tenascin expression in mucocutaneous diseases and related lesions of human oral mucosa.
Tiitta, O, et al.
Archives of Oral Biology, 40, 1039-1045 (1995)
Y Soini et al.
Journal of clinical pathology, 46(3), 218-221 (1993-03-01)
To determine the distribution of tenascin in normal and pathological bone marrow. 48 different bone marrow lesions were studied immunohistochemically using a monoclonal antibody to tenascin. Tenascin immunoreactivity was found in lesions with increased fibrosis and high numbers of reticular
Demonstration of tenascin-like immunoreactivity in rabbit corneal wounds.
Tervo, K, et al.
Acta Ophthalmologica, 67, 347-350 (1989)
Pleural mesotheliomas have an integrin profile distinct from visceral carcinomas.
Koukoulis, G K, et al.
Human Pathology, 28, 84-90 (1997)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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