MAB1904

Anti-Laminin-1 A&B chains Antibody

CHEMICON^®, rat monoclonal, AL-2

• 別名: laminin alpha1, laminin beta1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 製品名: Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2, clone AL-2, Chemicon^®, from rat
• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: AL-2, monoclonal
• 交差性: mouse
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NM_005559.2
• UniProtアクセッション番号: P25391; P55268
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Lama1(16772)

詳細

詳細

Laminins are secreted glycoproteins incorporated into cell-associated extracellular matrices and an integral part of the structural scaffolding in most animal tissues. The laminin protein is made up of three polypeptide chains; α, β and γ, accounting for its flexibility in connecting various molecules. When originally characterized, laminin-1 clone AL-2 reacted with Engelbreth-Holm-Swarm (EHS) mouse laminin A & B chains on the long arm near the cross region, (Skubitz, 1987). More recently, investigations, (Schèele, 2006) have shown that the clone binds laminin β1 under reducing conditions as well as native ELISAs and that the affinity is increased by the presence of the laminin α1 chain. Thus there is significant laminin β1 reactivity with clone AL-2.

特異性

Other species have not been tested.

Reacts with EHS mouse laminin A & B chains on the long arm near the cross region.

免疫原

Epitope: Near cross region

Laminin isolated from EHS mouse sarcoma.

アプリケーション

Research Category
細胞骨格

Research Sub Category
ECMタンパク質

Detect Laminin-1 A&B chains using this Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2 validated for use in ELISA, IF, IH(P), IP & WB.

Immunofluorescence:
An independent laboratory (Schèele, S., 2006) has verified anti-laminin clone AL-2 to work using immunofluorescence techniques on mouse muscle tissue, including both transgenic and wild-type tissues.

品質

Evaluated on a representative lot by Western blot on purified mouse laminin-1.

ターゲットの説明

~200 kDa

物理的形状

Protein G Purified

Format: Purified

Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.

保管および安定性

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable