MAB16200

Anti-MGMT Antibody, clone MT3.1

clone MT3.1, Chemicon^®, from mouse

• 別名: O-6-methylguanine-DNA Methyltransferase, Methylated-DNA--Protein-cysteine Methyltransferase
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: MT3.1, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Chemicon^®
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NM_002412.2
• UniProtアクセッション番号: P16455
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MGMT(4255)

詳細

詳細

O6-Methylguanine-DNA Methyltransferase (MGMT) is a ubiquitous DNA repair protein that removes O6-alkyl-guanine, primarily O6 methylguanine lesions from damaged DNA. It is a major contributor to cellular protection from the mutagenic, carcinogenic, and cytotoxic effects of DNA alkylation. The mechanism of MGMT action is based on the transfer of the alkyl group from the DNA to a unique acceptor cysteine residue in the protein, forming a stable thioether linkage. MGMT is frequently classified as a suicide protein. The repair capacity for O6- methylguanine is dependent on the number of MGMT molecules in the cell. There is a correlation between the occurrence of cancer in various tissues and the lack of the MGMT enzyme. High levels of MGMT result in reduced tumor events and resistance of tumors to alkylating agents.

特異性

MGMT is a 22 kDa DNA repair protein that is present in all normal tissues; however, in a subset of human tumours MGMT is completely absent. Thus, antibody MAB16200 may be helpful in utilizing MGMT as a marker of malignant or pre-malignant cells. Because MGMT repairs the potentially cytotoxic antitumour lesions induced in DNA by certain cancer chemotherapeutic alkylating agents (e.g. BCNU, CCNU, DTIC, procarbazinc, temozolomide), tumours lacking or having low levels of MGMT will predictably be responsive to drug therapy. Conversely, tumours with high MGMT levels will likely be drug resistant. The MGMT antibody may also be used to gauge the effectiveness of MGMT modulators, such as O-6-benzylguanine, which lead to rapid degradation of the inactivated protein.

免疫原

Made against recombinant human MGMT (O-6-methylguanine-DNA methyltransferase) expressed in E. coli.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
細胞周期、DNA複製及び修復

Flow Cytometry:
An independent laboratory used a previous lot on flow cytometry (Gerson, 1996).

Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.

Immunohistochemistry:
A previous lot of this antibody was used on paraffin embedded sections.

Optimal working dilutions must be determined by end user.

Use Anti-MGMT Antibody, clone MT3.1 (mouse monoclonal antibody) validated in FC, ICC,IHC(P), IP, WB to detect MGMT also known as O-6-methylguanine-DNA Methyltransferase, Methylated-DNA--Protein-cysteine Methyltransferase.

Useful for detection of MGMT in human tissues, tumours, cells, or xenograft lines. Gives weak cross-reactivity with MGMT from mouse, hamster and rat cells.

品質

Evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected MGMT on 10 µg of Jurkat lysates.

ターゲットの説明

22-25 kDa

物理的形状

Protein A purified

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB, pH 7.6, 0.25 M NaCl containing 0.1% sodium azide.

保管および安定性

Stable for 1 year at 2-8ºC from date of receipt.

アナリシスノート

Control
Positive Control: Tonsil tissue, HeLa cells, CEM-CCRF cells.
Negative Control: TK6 cells.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable