GR32L

Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2)

lyophilized, clone BuGR2, Calbiochem^®

• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: BuGR2, monoclonal
• 形状: lyophilized
• 含みます: ≤0.1% sodium azide as preservative
• 化学種の反応性: guinea pig, rabbit, rat, mouse, sheep
• 以下との反応禁止: amphibian, primate, avian
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• アイソタイプ: IgG2a
• 輸送温度: ambient
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Nr3C1(14815)

詳細

詳細

Anti-Glucocorticoid Receptor (Ab-2), mouse monoclonal, clone BuGR2, recognizes ~94-97 kDa glucocorticoid receptor in mouse liver. It is validated for WB, ICC, IP & IHC (Frozen & paraffin sections).

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-AgX-653 myeloma cells (see application references). Recognizes the ~94-97 kDa glucocorticoid receptor.

Recognizes the ~94-97 kDa glucocorticoid receptor in mouse liver tissue.

免疫原

Rat

partially purified, rat glucocorticoid receptor

アプリケーション

Frozen Sections (5 g/ml)
Immunoblotting (5 g/ml)
Immunocytochemistry (5 g/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 g/ml)

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from PBS, pH 7.2.

再構成

Resuspend the lyophilized antibody with 0.1 ml sterile PBS, pH 7.2. Be careful to reconstitute the entire contents of the vial; during shipment and handling, portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

アナリシスノート

Positive Control
mouse liver extract

その他情報

Does not cross react with androgen, progesterone, estrogen or mineralocorticoid receptors. Enzymatic digestion and immunoblot analysis has shown that this antibody reacts with the undigested 97 kDa glucocorticoid receptor, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid and DNA-binding chymotrypsin fragment. Immunocytochemical localization of the glucocorticoid receptor is observed in the cytoplasm and nucleus, even in the presence of hormone. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Hussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA94, 1521.
Pratt, W.B. and Toft, D.O., 1997. Endocrine Rev.18 306.
Schumacher, M., et al. 1996. Dev. Neurosci.18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci.18, 36.
Weigel, N.L. 1996. Biochem J.319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci.743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable