FCMAB100P

Milli-Mark Anti-Phospho-Erk1/2 Antibody (Thr202/Tyr204, Thr185/Tyr187)-PE

clone AW39R, Milli-Mark^®, from rabbit

• 別名: Erk1 ; ; Erk2 ; ; p44-MAPK ; ; p42-MAPK ; ; MAPK2 ; ; PRKM2 ; ; p41mapk ; ; PRKM1 ; ; p44 ; ; ERK2 ; ; P42MAPK ; ; ERK ; ; p42 ; ; MAPK1 ; ; ERT1 ; ; ERT2
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: PE
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: AW39R, monoclonal
• 化学種の反応性: mouse, rat
• 化学種の反応性（ホモロジーによる予測）: human (based on 100% sequence homology)
• メーカー／製品名: Milli-Mark^®
• テクニック: flow cytometry: suitable; western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_002736.3
• UniProtアクセッション番号: P28482
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation ((pThr202/pTyr204), (pThr185/pTyr187))
• 遺伝子情報: human ... MAPK3(5595)

詳細

詳細

Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).

特異性

Antibody recognizes Recognizes Erk 1 & 2 only when dually phosphorylated on its TxY activation motif.

免疫原

Epitope: Phosphorylated Thr185/Phosphorylated Tyr187

Phosphorylated recombinant peptide corresponding to amino acids surrounding the pTEpY motif in the activation loop of human phospho-Erk1/2, in which the Thr and Tyr residues are phosphorylated

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
MAPキナーゼ

This Milli-Mark Phospho-Erk1/2 antibody is validated for use in Flow & WB for the detection of the Milli-Mark Phospho-Erk1/2 protein.

品質

Evaluated by Flow Cytometry with Jurkat cells.

ターゲットの説明

42kDa and 44kDa Observed

物理的形状

Protein A purified

Purified Rabbit monoclonal supernatant conjugated to phycoerythrin in buffer containing PBS with 0.05% sodium azide

保管および安定性

Stable for 6 months at 2-8°C from date of receipt. Protect from light.

アナリシスノート

Control
Jurkat Cells

法的情報

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable