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Merck

EZHTNFA-150K

Millipore

Human TNF-α ELISA Kit

detects TNF-α in 50 μL serum, plasma or cell culture supernatent

別名:

Human TNF-A ELISA, TNF-A ELISA, TNF-Alpha, Tumour Necrosis Factor Alpha ELISA

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About This Item

UNSPSCコード:
41116158
NACRES:
NA.77

product name

Human TNF-α Conferma® ELISA Kit, kit sufficient for 96 wells

品質水準

化学種の反応性

human

包装

kit of 1 × 96 wells

パラメーター

50 μL sample volume (4hr assay)

assay range

linearity: 89-113%
(Serum, 101%)

linearity: 98-110%
(Plasma, 103%)

recovery range: 81-111%
(Plasma, 97%)

recovery range: 85-105%
(Serum,95%)

inter-assay cv: ≤7%
intra-assay cv: ≤3%
sensitivity: 1.65 pg/mL
standard curve range: 1.65-400 pg/mL

テクニック

ELISA: suitable

入力

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

アプリケーション

research use

検出方法

colorimetric (450nm/590nm)

輸送温度

wet ice

保管温度

2-8°C

詳細

TNF-α is a core biomarker for the study of inflammation, considered to be a major contributing factor to numerous diseases and pathological processes. The Conferma® ELISA for TNF-α uses highly characterized critical reagents assessed by LC-MS, RPLC-UV-MS and Surface Plasmon Resonance (SPR) to deliver ensure similarity and that the mAb / Standard relationship is maintained . Each assay is further anchored against a recombinant gold standard and a sample library to ensure strong finished kit performance lot to lot. Detailed information can be found in the Protocol & Assay Verification Report.

Research Category: Inflammation

Research Sub-Category:
Alzheimer′s Disease
Aging
Inflammation
Neurodegenerative Diseases
Metabolic Disease
Sepsis Viral Diseases
Cardiovascular Disease

特異性

This assay was tested for cross reactivity with other human cytokines like IL-1a, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17A, CCL3, CCL4, MCP-1, TNFα, GMCSF, VEGF. There is no cross-reactivity detected with any analyte.

アプリケーション

For the detection of TNF-α by ELISA in Human Serum & Plasma.

特徴および利点

Capture ELISA assay. Highly Verified. Suitable for 37 samples in Duplicate, with a 6 point Standard Curve, 3 Quality Controls and a Blank.

法的情報

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ピクトグラム

CorrosionExclamation markHealth hazard

シグナルワード

Danger

危険有害性の分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2

ターゲットの組織

Respiratory Tract

保管分類コード

8A - Combustible, corrosive hazardous materials

WGK

WGK 3


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