CBL251

Anti-Epithelial Specific Antigen Antibody, clone VU-1D9

clone VU-1D9, from mouse

• 別名: Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastro
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: VU-1D9, monoclonal
• 化学種の反応性: human
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_002345
• UniProtアクセッション番号: P16422
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Epithelial specific antigen, also known as epithelial cell adhesion molecule (Ep-CAM) and epithelial glycoprotein (EGP), may act as an interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Epithelial specific antigen plays a role in embryonic stem cells proliferation and differentiation, and up-regulates the expression of FABP5, MYC, and cyclins A and E. Epithelial specific antigen is highly and selectively expressed by undifferentiated embryonic stem cells (ESCs). Protein levels rapidly diminish as soon as ESCs differentiate. Also expressed in most epithelial cell membranes and on the surface of adenocarcinoma.

免疫原

NCI-H69 whole cells

アプリケーション

Research Category
細胞骨格

Research Sub Category
ECMタンパク質

Anti-Epithelial Specific Antigen Antibody, clone VU-1D9 is a highly specific mouse monoclonal antibody, that targets Epithelial Cell Marker & has been tested in western blotting, ICC & IHC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Epithelial Specific Antigen in A431 cells.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Epithelial Specific Antigen in human prostate adenocarcinoma cells.

品質

Evaluated by Western Blotting in MCF7 cell lyate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Epithelial Specific Antigen in 10 µg of MCF7 cell lyate.

ターゲットの説明

~40 kDa observed. The calculated molecular weight of this protein is 35 kDa; however, this protein will run higher due to glycosylation.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
MCF7 cell lyasate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable