おすすめの製品
由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
CHC5.9, monoclonal
化学種の反応性
bovine, amphibian, rat, pig, human
メーカー／製品名
Chemicon®
テクニック
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgM
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CLTC(1213)
特異性
This antibody reacts with the clathrin heavy chain from a diverse range of species. In nervous tissue it cross-reacts with another coated vesicle polypeptide of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may cross-react with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland). : Recognizes bovine, rat and amphibian
免疫原
Coated vesicles (clathrin) of bovine brain.
アプリケーション
Research Category
エピトープタグ抗体及び凡用抗体
エピトープタグ抗体及び凡用抗体
Research Sub Category
オルガネラ及び細胞マーカー
オルガネラ及び細胞マーカー
Anti-Clathrin Antibody, clone CHC5.9 is an antibody against Clathrin for use in WB, IH.
Marker for detection of receptor mediated endocytosis
Suitable for frozen sections
Western blotting KNOW SPECIES
Optimal working dilutions must be determined by the end user.
Suitable for frozen sections
Western blotting KNOW SPECIES
Optimal working dilutions must be determined by the end user.
物理的形状
Format: Purified
The antibody was purified by gel filtration and is presented as 100μg of lyophilysed material. Reconstitute with 1ml distilled water. Final buffer composition is PBS, pH 7.4.
保管および安定性
Maintain at 2-8°C for up to 12 months from date of receipt.
アナリシスノート
Control
Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.
Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
13 - Non Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
CBL188:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Hisako Furukawa et al.
Laboratory investigation; a journal of technical methods and pathology, 84(7), 828-835 (2004-05-04)
Prion protein (PrP) is known to accumulate in some non-neuronal tissues under conditions unrelated to prion diseases. The biochemical and biological nature of such accumulated PrP molecules, however, has not been fully evaluated. In this study, we established experimental myopathy
Molecular links between endocytosis and the actin cytoskeleton.
B Qualmann et al.
The Journal of cell biology, 150(5), F111-F116 (2000-09-07)
J Hirst et al.
Biochimica et biophysica acta, 1404(1-2), 173-193 (1998-08-26)
Clathrin and adaptors are components of clathrin-coated pits and vesicles. The AP-1 adaptor complex is associated with clathrin-coated vesicles budding from the TGN, while the AP-2 adaptor complex is associated with clathrin-coated vesicles budding from the plasma membrane. The clathrin
G Bruder et al.
Experimental cell research, 164(2), 449-462 (1986-06-01)
We have used a monoclonal antibody (CHC5.9) to identify clathrin (Mr 180,000; 'heavy chain') in coated vesicles, triskelion structures prepared in vitro and in high-speed supernatants (HSS) of cell homogenates from a variety of tissues and species (e.g., brain and
Nadezhda V Popova et al.
Journal of neurochemistry, 118(6), 988-998 (2011-07-14)
TPR-containing Rab8b-interacting protein (TRIP8b) is a brain-specific hydrophilic cytosolic protein that contains tetratricopeptide repeats (TPRs). Previous studies revealed interaction of this protein via its TPR-containing domain with Rab8b small GTPase, hyperpolarization-activated cyclic nucleotide-regulated channel (HCN) channels and G protein-coupled receptor
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）