ABT155

Anti-Dynamin related protein 1 (Drp1) Antibody

from rabbit, purified by affinity chromatography

• 別名: Dynamin-1-like protein, Dynamin family member proline-rich carboxyl-terminal domain less, Dymple, Dynamin-related protein 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 交差性: human, mouse
• 交差性（ホモロジーによる予測）: rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology), ox (based on 100% sequence homology)
• テクニック: immunocytochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_690029
• UniProtアクセッション番号: Q8K1M6
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Dnm1L(74006)

詳細

詳細

Dynamin related protein 1 (Drp1), also known as Dynamin-like protein 1, mediates outter mitochondrial membrane fission in mammilian cells. Like other members of the Dynamin superfamily of GTPases, Drp1 contains a N-terminal tripartate GTP-binding domain and exists as a T-shaped dimer with a head, leg, and stalk. The addition of GTP induces head and stalk rearrangement resulting in membrane constriction. Drp1 has been shown to affect mitochondrial morphology and places a significant role in mitochondrial and peroxisomal fission in mammilian cells. During apoptosis, Drp1 translocates from the cytosol to mitochondria and localizes to potential organelle division sites. Studies show Drp1 inhibition averts cell death, reinforcing to the critical role that mitochondrial fission plays in apoptosis.

特異性

This antibody recognizes Dynamin related protein 1 (Drp1) at the GSK3B interaction domain.

免疫原

Epitope: GSK3B interaction domain

KLH-conjugated linear peptide corresponding to the GSK3B interaction domain of mouse Dynamin related protein 1 (Drp1).

アプリケーション

Research Sub Category
オルガネラ及び細胞マーカー

アポトーシス-追加

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Dynamin related protein 1 in human embryonic stem cell H9 cells.

This Anti-Dynamin related protein 1 (Drp1) Antibody is validated for use in WB, IC for the detection of Dynamin related protein 1 (Drp1).

品質

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin related protein 1 (Drp1) on 10 µg of mouse embryonic stem cell lysate.

ターゲットの説明

~80kDa (isoform 2) & ~82 kDa (isoform 1) observed
5 isoforms are produced by alternative splicing: Isoform 1: 80 kDa, Isoform 2: 82 kDa, Isoform 3: 78 kDa, Isoform 4: 69 kDa, and Isoform 5: 24 kDa

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable