ABS82-AF488

Anti-VEGF Antibody, Alexa Fluor^™ 488 Conjugate

from rabbit, ALEXA FLUOR^™ 488

• 別名: Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: ALEXA FLUOR^™ 488
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: rat, human
• 化学種の反応性（ホモロジーによる予測）: nonhuman primates (based on 100% sequence homology)
• テクニック: immunocytochemistry: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_001020537
• UniProtアクセッション番号: P15692
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... VEGFA(7422)

詳細

詳細

VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic factor. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties, such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF also enhances permeabilization of blood vessels and plays a central role in the regulation of vasculogenesis. VEGF expression has been linked to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.

特異性

Other homologies: Bovine (85% sequence homology). Rat (70% sequence homology). Mouse (65% sequence homology).

This antibody recognizes VEGF at the N-terminus.

免疫原

Epitope: N-terminus.

KLH-conjugated linear peptide corresponding to human VEGF at the N-terminus.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
成長因子及び受容体

Anti-VEGF Antibody, Alexa Fluor^™ 488 Conjugate is an antibody against VEGF for use in Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VEGF in HeLa and A431 cells.
The unconjugated antibody (Cat. No. ABS82) is shown to be suitable also for Western blotting and immunohistochemistry (Paraffin) applications.

品質

Evaluated by Immunocytochemistry in NIH/3T3 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected VEGF in NIH/3T3 cells.

ターゲットの説明

~55 kDa observed.

物理的形状

Affinity purified

Purified rabbit polyclonal antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

法的情報

ALEXA FLUOR is a trademark of Life Technologies

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable