ABS433

Anti-IL-32 Antibody

from rabbit, purified by affinity chromatography

• 別名: Interleukin-32, IL-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, NK4, TAIF
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• テクニック: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• UniProtアクセッション番号: P24001
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... IL32(9235)

詳細

詳細

Interleukin-32 (IL-32), also known as Natural killer cells protein 4 (NK4) and Tumor necrosis factor alpha-inducing factor (TAIF), may play a role in innate and adaptive immune responses. IL-32 induces various cytokines such as TNFA/TNF-alpha, MIP-2 and IL-8, and activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. IL-32 has been shown to be induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFN gamma, and it has been suggested to play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis. IL-32 is different from other known cytokine families because it does not share sequence homology with them and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. IL-32 is selectively expressed in lymphocytes and expression is more prominent in immune cells than in non-immune cells. The expression of IL-32 increased after activation of T-cells by mitogens and after activation of NK cells by IL2/interleukin-2.

特異性

This antibody recognizes two isoforms at ~14 kDa and ~22 kDa at the C-terminus of IL-32.

免疫原

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human IL-32.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
発生シグナル伝達

Anti-IL-32 Antibody is a highly specific rabbit polyclonal antibody, that targets IL-32 & has been tested in western blotting, IHC & Immunofluorescence.

Immunohistochemistry Analysis: 10 µg/mL from a representative lot detected IL-32 in human spleen tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected IL-32 in human spleen cells.

品質

Evaluated by Western Blot in human spleen tissue lysate.

Western Blot Analysis: 5 µg/mL of this antibody detected IL-32 in 15 µg of human spleen tissue lysate.

ターゲットの説明

~14 kDa and ~22 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 5 isoforms between ~15 kDa and ~27 kDa

物理的形状

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Human spleen tissue lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable