ABS2235

Anti-TFF2

• 別名: Spasmolytic polypeptide, Trefoil factor 2
• UNSPSCコード: 12352203
• NACRES: NA.41
• クローン: N/A, polyclonal
• application: WB
• 化学種の反応性: mouse
• テクニック: western blot: suitable

特性

• 由来生物: rabbit
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: N/A, polyclonal
• 分子量: calculated mol wt 14.17 kDa; observed mol wt ~17 kDa
• 精製方法: using Protein A
• 化学種の反応性: mouse
• 化学種の反応性（ホモロジーによる予測）: human
• 包装: antibody small pack of 100 μg
• テクニック: western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: C-terminal
• タンパク質IDアクセッション番号: NP_033389
• UniProtアクセッション番号: Q03404
• 保管温度: 2-8°C
• 遺伝子情報: mouse ... Tff2(21785)

詳細

詳細

Trefoil factor 2 (UniProt: Q03404; also known as Spasmolytic polypeptide, TFF2) is encoded by the Tff2 (also known as Sml1, Sp) gene (Gene ID: 21785) in murine species. TFF2 is secreted into the mucus layer of the gastrointestinal tract of mammals where it stabilizes the mucin gel layer and stimulates migration of epithelial cells. It inhibits gastrointestinal motility and gastric acid secretion and possibly stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. It is also reported to stimulate epithelial restitution and restrain any associated inflammation. TFF2 levels are elevated in hypergastrinemic transgenic mice and reduced in gastrin-deficient mice. Gastrin treatment is shown to increase TFF2 promoter activity through cis-acting regions, containing CCAATA- and GC-rich enhancers. Pretreatment with gastrin/CCKB receptor antagonists is reported to abolish gastrin-dependent promoter activity. Inhibitor of PKC, MEK1, and PI-3 kinase are also shown to reduce gastrin-dependent TFF2 promoter activity. TFF2 levels are elevated in several pathological conditions, including gastric ulceration, duodenal ulceration, and Crohn s disease. In addition to its cytoprotective role, TFF2 also plays a role in the regulation of host immunity as evidenced by reduced inflammation in rat and mouse models of colitis following administration of recombinant TFF2. Higher expression of TFF2 is also observed in large tumors of the diffuse type. An increased prevalence of TFF2 expression is seen in tumors with advanced T and N stage and in patients with lymphatic and venous invasion. (Ref.: Dubeykovskaya, Z., et al. (2009). J. Biol. Chem. 284(6); 3650-3662; Tu, S., et al. (2007). Am. J. Physiol. 292; G1726-G1737; Dhar, DK., et al. (2003). Lab. Invest. 83(9); 1343-1352).

特異性

This rabbit polyclonal antibody detects Trefoil factor 2 (TFF2). It targets an epitope within 16 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of mouse Trefoil factor 2 (TFF2).

アプリケーション

Quality Control Applications

Evaluated by Western Blotting in Mouse stomach tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TFF2 in Mouse stomach tissue lysate.

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected TFF2 in mouse stomach tissue sections.

Western Blotting Analysis: A representative lot detected TFF2 in Western Blotting application (Tu, S., et. al. (2007). Am J Physiol Gastrointest Liver Physiol. 292; G1726-G1737).

Immunohistochemistry Applications: A representative lot detected TFF2 in Immunohistochemistry applications (Tu, S., et. al. (2007). Am J Physiol Gastrointest Liver Physiol. 292; G1726-G1737).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

物理的形状

Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide

保管および安定性

Recommended storage: +2°C to +8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable