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由来生物
rabbit
品質水準
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
rat, mouse, human
テクニック
immunofluorescence: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ALDH1A3(220)
詳細
Aldehyde dehydrogenase family 1 member A3 (EC 1.2.1.5; UniProt Q9JHW9; also known as Aldehyde dehydrogenase 6, RALDH-3, RalDH3, Retinaldehyde dehydrogenase 3) is encoded by the Aldh1a3 (also known as Aldh6, Raldh3) gene (Gene ID 56847) in murine species. All-trans-retinoic acid (atRA) is a small molecule metabolite of retinol, also known as vitamine A, that plays an important role in the growth and development of many major organ systems, including the postnatal eye growth. The three retinaldehyde dehydrogenases, RALDH1-3 or ALDH1 1-3, catalyze the irreversible oxidation of retinaldehyde to form atRA. While retinaldehyde is formed via the reversible oxidation of retinol catalyzed by retinol dehydrogenases (RDHs). The three RALDH enzymes cooperatively mediate atRA signaling during eye development through tightly regulated spatiotemporal expression patterns. In the postnatal human eye, RALDH catalytic activity can be detected in the choroid, retinal pigment epithelium (RPE), and retina. RALDH2 is most abundant in the choroid and RPE, moderate abundance in the retina, and in relatively low abundance in sclera. RALDH1 is most abundant in the choroid, in moderate abundance in the sclera, and substantially reduced in the retina and RPE. RALDH3 is most abundantly expressed during embryonic eye development, but not detectable in adult ocular fundal tissues.
免疫原
KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of mouse RalDH3.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Anti-RalDH3 (ALDH1A3), Cat. No. ABN427, is a highly specific rabbit polyclonal antibody that targets RalDH3/ALDH1A3 and has been tested in Immunofluorescence and Western Blotting.
Western Blotting Analysis: A 1:500 dilutioin from a representative lot detected RalDH3 in 10 µg of human retina tissue lysate.
Immunofluorescence Analysis: A 1:150 dilutioin from a representative lot immunostained trabecula analge of the developing eye in paraffin-embedded embryonic E15 Sprague Dawley (SD) rat head sections by fluorescent immunohistochemistry (Courtesy of Anna Ashton, University of Aberdeen).
Western Blotting Analysis: A 1:1,500 dilutioin from a representative lot detected RalDH3 in 50 µg of new born (postnatal P0) Sprague Dawley (SD) rat whole eye tissue lysate, but not in adult SD rat brain lateral cortex and pons tissue lysates (Courtesy of Anna Ashton, University of Aberdeen).
Immunofluorescence Analysis: A 1:150 dilutioin from a representative lot immunostained trabecula analge of the developing eye in paraffin-embedded embryonic E15 Sprague Dawley (SD) rat head sections by fluorescent immunohistochemistry (Courtesy of Anna Ashton, University of Aberdeen).
Western Blotting Analysis: A 1:1,500 dilutioin from a representative lot detected RalDH3 in 50 µg of new born (postnatal P0) Sprague Dawley (SD) rat whole eye tissue lysate, but not in adult SD rat brain lateral cortex and pons tissue lysates (Courtesy of Anna Ashton, University of Aberdeen).
品質
Evaluated by Western Blotting in mouse retina tissue lysate.
Western Blotting Analysis: A 1:500 dilutioin of this antibody detected RalDH3 in 10 µg of mouse retina tissue lysate.
Western Blotting Analysis: A 1:500 dilutioin of this antibody detected RalDH3 in 10 µg of mouse retina tissue lysate.
ターゲットの説明
~53 kDa observed. 55.98/56.03/56.04 kDa (human/mouse/rat) calculated (Met1 removed).
物理的形状
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABN427:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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