ABN306

Anti-MANF Antibody

from rabbit, purified by affinity chromatography

• 別名: Mesencephalic astrocyte-derived neurotrophic factor, arginine-rich mutatedin early stage tumor, ARMET, ARP
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: rat, mouse, human
• テクニック: immunohistochemistry: suitable; western blot: suitable
• UniProtアクセッション番号: P55145
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MANF(7873)

詳細

詳細

MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson’s disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.

特異性

This antibody does not cross-react with CDNF.

免疫原

KLH-conjugated linear peptide corresponding to human MANF.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経変性疾患

Detect the MANF protein using this Anti-MANF validated for use in WB & IHC.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected MANF in human midbrain and rat brain tissues.

品質

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected MANF in 15 µg/mL of PC12 cell lysate.

ターゲットの説明

~18 kDa observed. The calculated molecular weight is 21 kDa, however MANF has been shown as an ~18 kDa band in western blots. (Mizobuchi, N., et al. (2007). Cell Structure and Function. 32:41-50.). Uncharacterized bands may appear at ~25 kDa and ~100 kDa in some lysates.

物理的形状

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
PC12 cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable