ABN2290

Anti-LHFPL5

• 別名: LHFPL tetraspan subfamily member 5 protein, Lipoma HMGIC fusion partner-like 5 protein, Tetraspan membrane protein of hair cell stereocilia
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: N/A, polyclonal
• 分子量: calculated mol wt 24.2 kDa
• 交差性: human
• 交差性（ホモロジーによる予測）: monkey
• 包装: antibody small pack of 100 μL
• テクニック: immunocytochemistry: suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: C-terminal
• タンパク質IDアクセッション番号: NP_872354
• UniProtアクセッション番号: Q8TAF8
• 保管温度: -10 to -25°C
• 遺伝子情報: human ... LHFPL5(222662)

詳細

詳細

LHFPL tetraspan subfamily member 5 protein (UniProt: Q8TAF8; also known as Lipoma HMGIC fusion partner-like 5 protein, Tetraspan membrane protein of hair cell stereocilia) is encoded by the LHFPL5 (also known as TMHS) gene (Gene ID: 222662) in human. LHFPL5 is a multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. Its localization to the plasma membrane requires the presence of protocadherin related 15 (PCDH15). LHFPL5 is recognized as a critical component of the mechano-transduction complex in inner-ear hair cells. It is shown to physically interact with and stabilize transmembrane channel-like protein 1 (TMC1). The C-terminal half of LHFPL5 is involved in this interaction. The N-terminal half is involved in its dimerization and interaction with PCDH15. Mutations in LHFPL5 gene are known to cause autosomal recessive deafness that is characterized by non-syndromic sensorineural hearing loss from damage to the neural receptors of the inner ear. Knockout of LHFPL5 in murine models is shown to result in TMC1 reduction in the hair bundles of auditory cells. (Ref.: Yu, X., et al. (2020). Proc, Natl. Acad. Sci. USA. 117(47); 29894-29903).

特異性

This rabbit polyclonal antibody detects LHFPL tetraspan subfamily member 5 protein (LHFPL5). It targets an epitope within 20 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human LHFPL tetraspan subfamily member 5 protein (LHFPL5).

アプリケーション

Quality Control Applications

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LHFPL5 in HeLa cells.

Tested applications

Western Blotting Analysis: A representative lot detected LHFPL5 in Western Blotting applications (Yu, Z., et. al. (2020). Proc Natl Acad Sci USA. 117(47); 29894-29903).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LHFPL5 in HEK293 cells transfected with LHFPL5 (TMHS) protein.

Immunocytochemistry Analysis: A representative lot detected LHFPL5 in Immunocytochemistry applications (Yu, Z., et. al. (2020). Proc Natl Acad Sci USA. 117(47); 29894-29903).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

物理的形状

Rabbit polyclonal antibody in 0.02 mg/mL sodium azide.

保管および安定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1