ABN1477

Anti-nSR100 (SRRM4)

from rabbit

• 別名: Serine/arginine repetitive matrix protein 4, Medulloblastoma antigen MU-MB-2.76, Neural-specific serine/arginine repetitive splicing factor of 100 kDa, Neural-specific SR-related protein of 100 kDa, nSR100
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 交差性: mouse, human
• 包装: antibody small pack of 25 μg
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBIアクセッション番号: NP_919262
• UniProtアクセッション番号: A7MD48
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SRRM4(84530)

詳細

詳細

Serine/arginine repetitive matrix protein 4 (UniProt: A7MD48; also known as Medulloblastoma antigen MU-MB-2.76, Neural-specific serine/arginine repetitive splicing factor of 100 kDa, Neural-specific SR-related protein of 100 kDa, nSR100) is encoded by the SRRM4 (also known as KIAA1853) gene (Gene ID: 84530) in human. nSR100 is a nuclear protein that is specifically expressed in neuronal cells. It serves as a splicing factor that is specifically required for neural cell differentiation. It acts in conjunction with nPTB/PTBP2 by binding directly to its regulated target transcripts and promote neural-specific exon inclusion in many genes that function in neural cell differentiation. It is shown to regulate the splicing of protrudin gene (Zfyve27) transcripts in neuronal cells and promotes splicing of protrudin pre-mRNA so as to include a microexon (exon L) encoding seven amino acids in a neuron-specific manner. The resulting protein known as Protrudin-L promotes neurite outgrowth during neurogenesis. Depletion of nSR100 in Neuro2A cells is shown to impair neurite outgrowth, whereas its ablation in mouse embryos is reported to prevent differentiation of neuronal progenitors in the cortex and impair development of the central and peripheral nervous systems. (Ref.: Ohnishi, T et al. (2017). Sci. Rep. 7: 41130; Quesnel-Vallières, M et al. (2015). Genes Dev. 29(7); 746-759).

特異性

This rabbit polyclonal antibody detects Serine/arginine repetitive matrix protein 4 in human and mouse cells. It targets an epitope within 104 amino acids from the C-terminal half.

免疫原

GST/His-tagged recombinant frgment corresponding to 104 amino acids from the C-terminal half of human Serine/arginine repetitive matrix protein 4.

アプリケーション

Anti-nSR100 (SRRM4), Cat. No. ABN1477, is a highly specific rabbit polyclonal antibody that targets Serine/arginine repetitive matrix protein 4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected nSR100 (SRRM4) in mouse cerebral cortex and human cerebral cortex tissue sections.

品質

Evaluated by Western Blotting in human cerebellum tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected nSR100 (SRRM4) in 10 µg of human cerebellum tissue lysate.

ターゲットの説明

~68 kDa observed; 68.56 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Format: Purified

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): does not flash
• 引火点(℃): does not flash