おすすめの製品
由来生物
rabbit
品質水準
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
mouse, hamster, human
テクニック
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
hamster ... Cdc45(100689362)
human ... CDC45(8318)
mouse ... Cdc45(12544)
詳細
DNA replication protein Cdc45 (UniProt: Q4U3P7; also known as Cdc45) is encoded by the CDC45 gene (Gene ID: 100689362) in hamster. Cdc45 is a replication initiation protein that plays an essential role in the establishment and progression of the replication fork. It is required for formation of pre-initiation complexes at the G1/S transition and for activation of replication origins. Cdc45 associates with the heterohexameric Mcm2-7 helicase and the GINS complex (go-ichi-nii-san, Japanese for 5-1-2-3, for the subunits Sld5, Psf1, Psf2 and Pfs3) to form the CMG (Cdc45-Mcm2-7-GINS) complex. CMG represents the eukaryotic replicative DNA helicase that unwinds origins of replication and drives the replication fork. Cdc45 is also reported to interact with several other replication proteins, including DNA helicase B (DHB), Mcm10, Orc2, TopPB1, and DNA polymerases alpha, delta and epsilon. Expression of Cdc45 is associated with the proliferative status of the cell or tissue. Terminally differentiated and senescent cells do not display Cdc45 activity whereas highly proliferative cells express high levels of Cdc45.
特異性
This rabbit polyclonal antibody detects Cdc45 in Human and Murine cells.
免疫原
His-tagged full length hamster recombinant Cdc45.
アプリケーション
Anti-Cdc45, Cat. No. ABE2902, is a highly specific rabbit polyclonal antibody that targets DNA replication protein Cdc45 and has been tested for use in Western Blotting.
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Cdc45 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: A representative lot detected Cdc45 in asynchronous, go synchronized and G1 released CHO cells, immunoprecipitated CDC45 from CHO cells, HeLa and NIH3T3 (Wong, P.G., et. al. (2011). PLoS One. 6(3):e17533).
Western Blotting Analysis: A representative lot detected Cdc45 in asynchronous, go synchronized and G1 released CHO cells, immunoprecipitated CDC45 from CHO cells, HeLa and NIH3T3 (Wong, P.G., et. al. (2011). PLoS One. 6(3):e17533).
品質
Evaluated by Western Blotting in CHO cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Cdc45 in 10 µg of CHO cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Cdc45 in 10 µg of CHO cell lysate.
ターゲットの説明
~65 kDa observed; 65.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Format: Purified
その他情報
Concentration: Please refer to lot specific datasheet.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABE2902:
試験成績書（COA）
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