ABE2868M

Anti-SOX9 Antibody

rabbit polyclonal

• 別名: Transcription factor SOX-9
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: polyclonal
• application: ICC; IHC; WB
• 化学種の反応性: rat, mouse, human
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable

特性

• 製品名: Anti-Sox-9, from rabbit
• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: unpurified
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: rat, mouse, human
• 包装: antibody small pack of 25 μL
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_000337
• UniProtアクセッション番号: P48436
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SOX9(6662)

詳細

詳細

Transcription factor SOX-9 (UniProt P48436; also known SOX-9) is encoded by the SOX9 gene (Gene ID 6662) in human. SOX family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences encoding for the HMG-box domains that mediate sequence-specific DNA binding. SOX genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of SOX genes cluster at about 40 genomic loci and at least 30 SOX genes have been identified. SOX-9 contains one HMG box DNA-binding domain and acts as a key regulator of developmental processes, including male sex determination, normal skeletal development, chondrogenesis, neurogenesis, and neural crest development. SOX-9 is acts downstream of sonic hedgehog signaling and is shown to be essential for the maintenance of multipotent neural stem cells in embryonic and adult central nervous system (CNS). It is not detected in neuroblasts of the adult subependymal zone (SEZ); however, it is expressed by transit amplifying cells and ependymal cells in the same region. SOX-9 is also shown to be essential for the ′switch′ from neurogenesis to gliogenesis in caudal regions of the developing CNS. SOX-9 up-regulation is reported in cancers of various origins, including breast, colon, prostate, and lung, where its expression correlates with malignancy and patient survival. (Ref.: Scott, CE et al. (2010). Nat. Neurosci. 13(10): 1181-89).

特異性

This rabbit ployclonal antibody detects SOX-9 in human, mouse, and rat cells. It targets an epitope with in 24 amino acids from the C-terminal end. This antibdy does not recognize Sox10.

免疫原

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal end of human Sox-9.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Anti-Sox-9, Cat. No. ABE2868, is a highly specific rabbit polyclonal antibody that targets Transcription factor SOX-9 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected SOX-9 in 10 µg of HepG2 cell lysate.

Western Blotting Analysis: A representative lot detected SOX-9 in Western Blotting applications (Augereau, C., et. al. (2016). Hum Mol Genet. Epub ahead of print).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected SOX-9 in HEK293T cells transfected with Sox-9 (Courtesy of Dr. Patrick Jacquemin atUniversité catholique de Louvain).

Immunofluorescence Analysis: A representative lot detected SOX-9 in PFA-fixed, paraffin-embedded pancreatic sections from an adult mouse; ductus (Augereau, C., et. al. (2016). Hum Mol Genet. 25(22):5017-5026).

Immunohistochemistry Analysis: A representative lot detected SOX-9 in PFA-fixed, paraffin-embedded liver (left) and pancreatic sections (right) of E14.5 mouse embryo, PFA-fixed, paraffin-embedded intestine sections of E14.5 mouse embryo (Augereau, C., et. al. (2016). Hum Mol Genet. 25(22):5017-5026).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SOX-9 in e14.5 mouse liver and e14.5 mouse pancreas tissues.

Immunocytochemistry Analysis: A representative lot detected SOX-9 in PFA-fixed 3T3 cells overexpressing Sox9 (Augereau, C., et. al. (2016). Hum Mol Genet. 25(22):5017-5026).

品質

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected SOX-9 in 10 µg of L6 cell lysate.

ターゲットの説明

~65 kDa observed; 56.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Unpurified

Format: Unpurified

Rabbit polyclonal antiserum with 0.02% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable