ABE281

Anti-EZH1 Antibody

from rabbit, purified by affinity chromatography

• 別名: Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: polyclonal
• application: IHC; WB
• 化学種の反応性: human, mouse
• テクニック: immunohistochemistry: suitable; western blot: suitable
• citations: 6

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human, mouse
• 化学種の反応性（ホモロジーによる予測）: rat (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• テクニック: immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_001982
• UniProtアクセッション番号: Q92800
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EZH1(2145)

詳細

詳細

Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH1 is the catalytic subunit of the PRC2/EED-EZH1 complex. This complex methylates Lys-27 of histone H3, which leads to transcriptional repression of the affected target gene. EZH1 is required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in maintaining the identity of embryonic stem cells.

特異性

Other homologies: Porcine (94% sequence homology).

This antibody potentially recognizes all isoforms of EZH1.

免疫原

KLH-conjugated linear peptide corresponding to human EZH1.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
ヒストン修飾タンパク質

Detect the EZH1 protein using this Anti-EZH1 validated for use in IHC & WB.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected EZH1 in human lung and kidney tissues.

品質

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected EZH1 in 10 µg of mouse embryonic stem cell lysate.

ターゲットの説明

~85 kDa observed. Uniprot states 5 isoforms for EZH1. This antibody potentially recognizes all isoforms. Isoform A is 85,271 Daltons. Isoform B is 86,025 Daltons. Isoform C is 80,699 Daltons. Isoform D is 76,952 Daltons. Isoform E is 68,961 Daltons. (Margueron, 2008).

関連事項

Replaces: 04-1047

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Mouse embryonic stem cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable