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Key Documents

安全性情報

ABE2621

Sigma-Aldrich

Anti-CEP164

from rabbit

別名:

Centrosomal protein of 164 kDa

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

rabbit

品質水準

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

polyclonal

化学種の反応性

human

テクニック

immunocytochemistry: suitable
western blot: suitable

アイソタイプ

IgG

NCBIアクセッション番号

UniProtアクセッション番号

輸送温度

ambient

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... CEP164(22897)

詳細

Centrosomal protein of 164 kDa (UniProt: Q9UPV0; also known as Cep164) is encoded by the CEP164 (also known as KIAA1052, NPHP15) gene (Gene ID: 22897) in human. Cep164 is expressed in several cell lines and it plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Cep164 localizes specifically to very distally located appendage structures on the mature centriole from which PC formation originates and it persists at centrioles throughout mitosis. Hence, it can serve as a marker for distal appendages on mature centrioles or basal bodies. Cep164 also plays a vital role in G2/M checkpoint and nuclear divisions. It is a key participant in the DNA damage-activated ATR/ATM signaling cascade and is required for the proper phosphorylation of H2AX, replication protein A, and check point kinases 1 and 2. Its phosphorylation at Ser186 is induced upon DNA-damage caused by treatment with IR irradiation, UV irradiation, hydroxyurea or amphidicolin. Mutations in CEP164 gene are known to cause nephronophthisis, a disease characterized by blindness in childhood, facial dysmorphism, bronchieclasis, liver failure and progression to end-stage renal failure.

特異性

This polyclonal antibody detects Centrosomal protein of 164 kDa in human. It targets an epitope within the first 298 amino acids from the N-terminal region.

免疫原

Epitope: N-terminus
His-taggged recombinant fragment of the first 298 amino acids from human Centrosomal protein of 164 kDa (CEP164).

アプリケーション

Research Category
エピジェネティクス及び核内機能分子
Anti-CEP164, Cat. No. ABE2621, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 164 kDa and has been tested in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected CEP164 in U2OS cells and hTERT-RPE1 cells that were serum starved for 48 hours (Graser, S., et. al. (2007). J Cell Biol. 179(2):321-30; Sonnen, K.F., et. al. (2012). Biol Open. 1(10):965-76).

Western Blotting Analysis: A representative lot detected CEP164 in total lysates of HeLa S3, U2OS, and 293T cells as well as 293T cells overexpressing myc-Cep164 (Graser, S., et. al. (2007). J Cell Biol. 179(2):321-30).

品質

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CEP164 in 10 µg of U2OS cell lysate.

ターゲットの説明

~200 kDa observed; 164.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein A purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

ABE2621:


試験成績書(COA)

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Thao P Phan et al.
Genes & development, 36(11-12), 718-736 (2022-07-01)
Centrosomes are microtubule-organizing centers comprised of a pair of centrioles and the surrounding pericentriolar material. Abnormalities in centriole number are associated with cell division errors and can contribute to diseases such as cancer. Centriole duplication is limited to once per

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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