由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human
テクニック
ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... HDAC4(9759)
詳細
Histone deacetylation acts a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Transcription is controlled in part by the dynamic acetylation and deacetylation of histone proteins. Histone deacetylases (HDACs) are recruited to specific promoters or chromosomal domains by association with DNA-binding proteins. HDAC4 is a histone deacetylase which deacetylates lysines on the core histones. HDAC4 accumulates in the nuclei of myotubes, suggesting involvement in muscle differentiation. HDAC4 does not bind DNA directly but interacts with the myocyte enhancer factors MEF2A, MEF2C, and MEF2D. HDAC4 also interacts with the breast cancer associated transcriptional repressor PLU-1/JARID1B, the Nr2c1/TR2 nuclear receptor, as well as the 14-3-3 chaperone protein, in a phosphorylation dependent manner.
特異性
This antibody recognizes HDAC4 at the C-terminus.
免疫原
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human HDAC4.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン
エピジェネティクス
ヒストン
エピジェネティクス
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected HDAC4 in HeLa and A431 cells.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Laboratory of Dr. Edward Seto, Moffitt Cancer Center)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Laboratory of Dr. Edward Seto, Moffitt Cancer Center)
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Laboratory of Dr. Edward Seto, Moffitt Cancer Center)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Laboratory of Dr. Edward Seto, Moffitt Cancer Center)
Use Anti-HDAC4 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IP, ChIP to detect HDAC4 also known as Histone deacetylase 4, HD4.
品質
Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected HDAC4 on 10 µg of HepG2 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected HDAC4 on 10 µg of HepG2 cell lysate.
ターゲットの説明
~125 kDa observed
関連事項
Replaces: 04-232
物理的形状
Unpurified
Rabbit polyclonal serum containing 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
HepG2 cell lysate
HepG2 cell lysate
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABE262:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Panobinostat reduces hypoxia-induced cisplatin resistance of non-small cell lung carcinoma cells via HIF-1? destabilization.
Fischer, C; Leithner, K; Wohlkoenig, C; Quehenberger, F; Bertsch, A; Olschewski et al.
Molecular Cancer null
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