ABE1975

Anti-Srg3/BAF155 Antibody

serum, from rabbit

• 別名: SWI/SNF complex subunit SMARCC1, BAF155, BRG1-associated factor 155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SWI3-related protein
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: human, mouse
• テクニック: ChIP: suitable; western blot: suitable
• NCBIアクセッション番号: NP_033237
• UniProtアクセッション番号: P97496
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SMARCC1(6599)

詳細

詳細

SWI/SNF complex subunit SMARCC1 (UniProt P97496; also known as BAF155, BRG1-associated factor 155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SWI3-related protein) is encoded by the Smarcc1 (also known as Baf155, Srg3) gene (Gene ID 20588) in murine species. SMARCC1/BAF155/SRG3 is a core component of the SWI/SNF complex that coordinates the expression of specific genes crucial for germinal center (GC) formation, including Blimp-1 and Bcl-6. The SWI/SNF is a chromatin-remodeling complex that drives conformational changes of nucleosomes in an ATP-dependent manner. SWI/SNF complex is required for the differentiation of GC B and T follicular helper (Tfh) cells. Smarcc1/Baf155/Srg3-knockout mice showed impaired differentiation of GC B and Tth cells in response to T cell-dependent antigen challenge. The SWI/SNF complex consists of at least 12 different subunits, including BRG1 with ATPase activity and SMARCC1/BAF155/SRG3 that controls the complex stability. SMARCC1/BAF155/SRG3 contains an N-terminal acidic region (23% Asp/Glu content from a.a. 211-441), a DNA-binding SWIRM domain (a.a. 448-545), a histone-interacting SANT domain (a.a. 617-668), a leucine-zipper motif (a.a. 899-942), and a C-terminal glutamine- and proline-rich region (a.a. 768-862 & 977-1104).

特異性

Reacts with both mouse and human Srg3/BAF155 proteins. Reproted to exhibit cross-reactivity toward a ~170 kDa murine ortholog of human BAF170 protein (Jeon, S.H., et al. (1997). J. Exp. Med. 185(10):1827-1836). Expected to react with also murine Srg3/Smarcc1 spliced isoform 2 reported by UniProt (UniProt P97496-2).

免疫原

Epitope: C-terminal region.

GST-tagged recombinant mouse Srg3/BAF155 C-terminal fragment.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
エピジェネティクス

Anti-Srg3/BAF155 Antibody is an antibody against Srg3/BAF155 for use in Western Blotting, Chromatin Immunoprecipitation (ChIP).

Western Blotting Analysis: A representative lot detected Srg3 immunoprecipitated from the nuclear extracts of human Raji and murine BAL17 B cells (Choi, J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(7):E718-727).
Western Blotting Analysis: A representative lot detected the ~155 kDa target Srg3 band and a ~170 kDa cross-reactive band in murine thymus and lymph node lysates. The ~170 kDa band likely represents the murine ortholog of human BAF170 protein (Jeon, S.H., et al. (1997). J. Exp. Med. 185(10):1827-1836).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Srg3 occupancy at the proximal promoter and BRE (Bcl-6 responsive element) regions of the Blimp-1 locus in germinal center (GC) B cells, but not in plasma B cells (PCs) (Choi, J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(7):E718-727).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Srg3 occupacy at the Blimp-1 locus using chromatin preprations from untreated murine BAL17 B lymphoma cells, but not Brg1/Srg3-depleted BAL17 cells following siRNAs treatment (Choi, J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(7):E718-727).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Srg3 and its associated proteins from the nuclear extracts of human Raji and murine BAL17 B cells (Choi, J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(7):E718-727).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated a ~195 kDa SWI2-like protein with the ~155 kDa Srg3 protein from murine thymus cell extracts (Jeon, S.H., et al. (1997). J. Exp. Med. 185(10):1827-1836).

品質

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Srg3/BAF155 in 10 µg of K562 cell lysate.

ターゲットの説明

~155 kDa observed. 122.9 kDa (human and mouse SRG3) and 120.1 kDa (mouse spliced isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

物理的形状

Unpurified

Rabbit polyclonal antibody serum with 0.05% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable