ABE1317

Anti-phospho Cyclin-dependent kinase 7 (Thr170) Antibody

from rabbit, purified by affinity chromatography

• 別名: Cyclin-dependent kinase 7, 39 kDa protein kinase, p39 Mo15, CDK-activating kinase 1, Cell division protein kinase 7, Serine/threonine-protein kinase 1, TFIIH basal transcription factor complex kinase subunit
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse, human, rat
• テクニック: ChIP: suitable; western blot: suitable
• NCBIアクセッション番号: NP_001790
• UniProtアクセッション番号: P50613
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pThr170)
• 遺伝子情報: human ... CDK7(1022)

詳細

詳細

Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin protein and they mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Cyclin-dependent kinase 7 (CDK7), also known as Cyclin-dependent kinase 7, or 39 kDa protein kinase (p39-Mo15), CDK-activating kinase 1, Cell division protein kinase 7, Serine/threonine-protein kinase 1, or TFIIH basal transcription factor complex kinase subunit, and encoded by the gene CDK7/CAK/CAK1/CDKN7/MO15/STK1, is a serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CDK7 is inactivated by phosphorylation, thus Cyclin-dependent kinase 7 (Thr107) is an inactive CDK7 protein.

免疫原

Epitope: Phosphorylated T107

Linear peptide corresponding to human phosphorylated phospho Cyclin-dependent kinase 7 (Thr170).

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
核受容体

This Anti-phospho Cyclin-dependent kinase 7 (Thr170) antibody is validated for use in western blotting & ChIP for the detection of phospho Cyclin-dependent kinase 7 (Thr170).

Western Blotting Analysis: A representative lot detected phospho Cyclin-dependent kinase 7 (Thr170) in Cdk7 and Csk1 kinase (Larochelle, S., et al. (2012). Nature Struc. Mole. Biol. 19(11):1108-1115).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho Cyclin-dependent kinase 7 (Thr170) in HCT116 cell lysate treated with doxorubicin (Larochelle, S., et al. (2012). Nature Struc. Mole. Biol. 19(11):1108-1115).

品質

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected phospho Cyclin-dependent kinase 7 (Thr170) in 10 µg of A431 cell lysate.

ターゲットの説明

~ 39 kDa observed

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable