ABC467

Anti-phospho BAD Antibody (Ser155)

from rabbit, purified by affinity chromatography

• 別名: Bcl2 antagonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-XL/Bcl-2-associated death promoter
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: bovine (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), canine (based on 100% sequence homology)
• テクニック: immunoprecipitation (IP): suitable; inhibition assay: suitable (peptide); western blot: suitable
• NCBIアクセッション番号: NP_031548
• UniProtアクセッション番号: Q92934
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: phosphorylation (pSer155)
• 遺伝子情報: human ... BAD(572)

詳細

詳細

Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118, Ser-134, and Ser-155 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.

免疫原

Epitope: BH3 domain

KLH-conjugated linear peptide corresponding to the BH3 domain of human phospho BAD (Ser155).

アプリケーション

Research Category
アポトーシス及び癌

Research Sub Category
アポトーシス-追加

Anti-phospho BAD (Ser155) is an antibody against phospho BAD (Ser155) for use in WB, PIA, IP.

Peptide Inhibition Analysis: A 1:100 dilution from a representative lot blocked phospho BAD (Ser155) in Forskolin treated PC12 cell lysates.
Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated phospho BAD (Ser155) in 0.5 mg of Forskolin treated PC12 cell lysate.

品質

Evaluated by Western Blotting in Forskolin treated PC12 cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected phospho BAD (Ser155) in 10 µg of Forskolin treated PC12 cell lysate.

ターゲットの説明

~20 kDa observed. Uncharacterized band(s) may appear in some lysates.

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable