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由来生物
rabbit
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
分子量
calculated mol wt 550.85 kDa
observed mol wt ~60 kDa
精製方法
using protein G
交差性
human
交差性（ホモロジーによる予測）
mouse
包装
antibody small pack of 100
テクニック
electron microscopy: suitable
western blot: suitable
アイソタイプ
IgGκ
エピトープ配列
N-terminal half
タンパク質IDアクセッション番号
UniProtアクセッション番号
保管温度
-10 to -25°C
遺伝子情報
human ... MUC2(4583)
特異性
This rabbit polyclonal antibody detects D3 domain of Mucin-2. It targets an epitope within D3 domain from the N-terminal half.
免疫原
His-tagged recombinant fragment corresponding to 402 amino acids from the D3 domain of human Mucin-2.
アプリケーション
Quality Control Testing
Evaluated by Western Blotting with Human Muc2-D3 protein.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Human Muc2-D3 protein.
Tested Applications
Electron Microscopy: A representative lot detected Human Mucin-2 protein in Electron Microscopy applications (Javitt, G., et. al. (2020). Cell.183(3): 717-729).
Western Blotting Analysis: A representative lot detected Human Mucin-2 protein in Western Blotting applications (Javitt, G., et. al. (2020). Cell. 183(3): 717-729).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with Human Muc2-D3 protein.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Human Muc2-D3 protein.
Tested Applications
Electron Microscopy: A representative lot detected Human Mucin-2 protein in Electron Microscopy applications (Javitt, G., et. al. (2020). Cell.183(3): 717-729).
Western Blotting Analysis: A representative lot detected Human Mucin-2 protein in Western Blotting applications (Javitt, G., et. al. (2020). Cell. 183(3): 717-729).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
ターゲットの説明
Mucin-2 (UniProt: Q02817; also known as MUC-2, Intestinal Mucin-2) is encoded by the MUC2 (also known as SMUC) gene (Gene ID: 4583) in human. Mucin-2 (MUC2) is a secreted protein that coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. It provides a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. Mucin-2 is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to produce the mature form. It exists as a disulfide-linked homotrimer that can be highly O-glycosylated. It contains four VWFD domains and two VWFC domains. Mucin-2 undergoes proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer that allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria. The numerous O-glycans on the Mucin-2 are reported to serve as nutrients for the bacteria, serve as attachment sites, and probably contribute to the selection of the species-specific colon flora. Reduced levels of Mucin-2 have been linked to the early stage of colorectal cancer development, which is caused by methylation of the MUC2 promoter. This polyclonal antibody detects D3 domain of mucin-2, which is localized in amino acids 858-1259. (Ref.: Wi, D-H., et al. (2021). BMB Rep. 54(7); 344-355; Javitt, G., et al. (2020). Cell. 183(3); 717-729; Javitt, G., et al. (2019). J. Mol. Biol. 431(19); 3740-3752; Johansson, MEV., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(Suppl. 1); 4659-4665).
物理的形状
Purified rabbit polyclonal antibody in PBS without azide.
再構成
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABC2044-25UG:
ABC2044-100UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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