おすすめの製品
由来生物
rabbit
品質水準
抗体製品の状態
affinity purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
rat, human
メーカー／製品名
Chemicon®
テクニック
immunocytochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... SEMA3F(6405)
特異性
Recognizes Semaphorin 3F (Sema3F), recombinant and purified protein. By Western blot the antibody recognizes a protein of 90 kDa in total extracts from tissue culture cells producing Sema3F. Semaphorin 3F is expressed ubiquitously with higher levels in lung, brain, spleen, muscle and heart.
This antibody may also react with mouse because of extremely high sequence conservation in the SEM3F protein.
免疫原
Synthetic peptide from human Semaphorin 3F (Catalog number AG363). (TPWTQTQAVRFRFSRATDGALRPM).
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
成長円錐及び軸策ガイダンス
成長円錐及び軸策ガイダンス
Anti-Semaphorin 3F Antibody is an antibody against Semaphorin 3F for use in IC & WB.
Western blot: 0.5-1.0 μg/mL using ECL. Suggested antibody dilution buffer is TBST (10 mM Tris-HCl, pH 8.0, 150 mM NaCl with 0.05% Tween 20). Suggested incubation is for two hours at room temperature or overnight at 2-8°C.
Immunocytochemistry.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry.
Optimal working dilutions must be determined by the end user.
ターゲットの説明
90 kDa
関連事項
Replaces: AB5471
物理的形状
ImmunoAffinity Purified
Affinity Purified immunoglobulin. Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
保管および安定性
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
アナリシスノート
Control
Embryonic brain tissue, neural growth cones
Embryonic brain tissue, neural growth cones
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB5471P:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Endogenous axon guiding chemorepulsant semaphorin-3F inhibits the growth and metastasis of colorectal carcinoma.
Wu, F; Zhou, Q; Yang, J; Duan, GJ; Ou, JJ; Zhang, R; Pan, F; Peng, QP; Tan, H; Ping et al.
Clinical cancer research : an official journal of the American Association for Cancer Research null
Expression of class 3 semaphorins and their receptors in human breast neoplasia.
Carolyn A Staton,Lucy A Shaw,Manoj Valluru,Leslie Hoh,Ivan Koay,Simon S Cross et al.
Histopathology null
Identification of genes differentially expressed in rat alveolar type I cells
Dahlin, Katherine, et al.
American Journal of Respiratory Cell and Molecular Biology, 31, 309-316 (2004)
Alexander Fassold et al.
Arthritis and rheumatism, 60(10), 2892-2901 (2009-10-01)
In inflammatory lesions, sympathetic nerve fibers disappear soon after the start of inflammation. We identified sympathetic nerve repellents as possible causal agents in rheumatoid arthritis (RA). On nerve terminals, repellent factors bind to neuropilin-2 and its coreceptor. The aim of
Benoit Favier et al.
Blood, 108(4), 1243-1250 (2006-04-20)
Neuropilin 2 (NRP2) is a receptor for the vascular endothelial growth factor (VEGF) and the semaphorin (SEMA) families, 2 unrelated ligand families involved in angiogenesis and neuronal guidance. NRP2 specifically binds VEGF-A and VEGF-C, although the biological relevance of these
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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