AB3265

Anti-Actin γ Antibody

serum, Chemicon^®

• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: sheep
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: mouse, human, rat
• メーカー／製品名: Chemicon^®
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NM_001614.2
• UniProtアクセッション番号: P63261
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ACTG1(71)

詳細

詳細

Actin is a 43kDa highly conserved structural protein. There are three main actin isotypes (alpha,beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. {Pardo et al, 1983, Cell 32:1093-1103 & Otey, CA et al 1986 J Cell Biol 102:1726-1737, Bassell et al, 1998, J Neurosci 18:251-265 {available online}. Furthermore, there are at least six actin isoforms in vertebrates: cardiac alpha-actin and skeletal-alpha actin (expressed in striated muscle), vascular alpha-actin and enteric gamma-actin (expressed in smooth muscle), and cytoplasmic beta- and gamma-actins.{http://www.cbil.upenn.edu/MTIR/gammaActin_E-toc.html}

特異性

Recognizes actin, gamma. Specificity for gamma actin determined by 2D gel electrophoresis.

免疫原

Epitope: gamma

Synthetic peptide corresponding to the amino terminus of gamma actin {EEEIAALVIDNGSGGSG} conjugated to KLH.

アプリケーション

Research Sub Category
細胞骨格

Anti-Actin Antibody, γ is an antibody against Actin for use in IC, IH & WB.

Research Category
Cell Structure

Western Blot: 1:500-1:2,000 or greater; non-specific bands can occur when primary antibody is overloaded. Blocking with purified milk proteins preferred over commerical concentrated milk solutions. Detects a 42-43kDa band. Specificity for gamma actin determined by 2D gel electrophoresis.

Immunocytochemistry: 1:500-1:2,000. Cultured cells fixed with PFA, then permeabilized with methanol.

Immunohistochemistry: 1:500-1:2,000. Tissues frozen and then fixed with PFA followed by Triton permeabilisation. 10% buffered formalin fixed tissues have also been used.

Optimal working dilutions must be determined by the end user.

ターゲットの説明

42-43kDa

物理的形状

Unpurified

Serum. Liquid, containing no preservatives.

保管および安定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

アナリシスノート

Control
Smooth muscle

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids