AB2302

Anti-GAPDH Antibody

chicken polyclonal

• 別名: glyceraldehyde 3-phosphate dehydrogenase, aging-associated gene 9 protein, glyceraldehyde-3-phosphate dehydrogenase
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 製品名: Anti-GAPDH Antibody, from chicken, purified by affinity chromatography
• 由来生物: chicken
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 交差性: human, mouse, horse, rat
• 交差性（ホモロジーによる予測）: canine (based on 100% sequence homology), equine (based on 100% sequence homology)
• 包装: antibody small pack of 25 μg
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_002037
• UniProtアクセッション番号: P04406
• 輸送温度: ambient; wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GAPDH(2597) ; mouse ... Gapdh(14433)

詳細

詳細

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).

特異性

This antibody recognizes GAPDH.

免疫原

Epitope: WARS interacting region

KLH-conjugated linear peptide corresponding to the WARS interacting region of human GAPDH.

アプリケーション

Research Category
代謝

Anti-GAPDH Antibody detects level of GAPDH & has been published & validated for use in WB.

Western Blot (SNAP ID) Analysis: 0.125 µg/ml from a previous lot detected GAPDH on 10 µg of NIH/3T3 and PC12 cell lysates.

品質

Evaluated by Western Blot in HEK293 and PC12 cell lysate.

Western Blot Analysis: 0.0625 µg/ml of this antibody detected GAPDH on 10 µg of HEK293 and PC12 cell lysate.

ターゲットの説明

~ 38 kDa

物理的形状

Affinity purified

Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
HEK293 cell lysate

その他情報

Concentration: Please refer to lot specific datasheet.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable