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由来生物
rabbit
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
rat, human
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... BDNF(627)
関連するカテゴリー
特異性
Brain Derived Neurotrophic Factor (BDNF). By dot blot, less than 1% cross-reactivity against NGF, NT3 or NT4.
免疫原
Recombinant Human Brain Derived Neurotrophic Factor (BDNF).
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経化学・ニューロトロフィン
神経炎症及び疼痛
神経化学・ニューロトロフィン
神経炎症及び疼痛
Anti-Brain Derived Neurotrophic Factor Antibody detects level of Brain Derived Neurotrophic Factor & has been published & validated for use in ELISA, WB, IH.
Immunohistochemistry: 5-10μg/mL (see suggested protocol).
Immunoblotting: 5-10μg/mL. BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4C. BDNF migrates as 18kDa, with a homodimer at ~27-30kDa. 1:1,000
ELISA: 5-10μg/mL
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Optimal working dilutions must be determined by end user.
Immunoblotting: 5-10μg/mL. BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4C. BDNF migrates as 18kDa, with a homodimer at ~27-30kDa. 1:1,000
ELISA: 5-10μg/mL
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Optimal working dilutions must be determined by end user.
関連事項
Replaces: AB1534P
物理的形状
Format: Purified
Immunoglobulin fraction lyophilized from PBS, with no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL).
保管および安定性
Maintain lyophilized material at -20 to -70°C for up to one year after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Glycerol (ACS grade or better) can be added (1:1) for additional stability.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
11 - Combustible Solids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB1779SP:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Effects of BDNF infusion on the axon terminals of locus coeruleus neurons of aging rats.
Sadamu Nakai, Wataru Matsunaga, Yoshiyuki Ishida, Ken-ichi Isobe, Tetsuya Shirokawa
Neuroscience Research null
E M Nikulina et al.
Neuroscience, 212, 38-48 (2012-04-24)
Intermittent social defeat stress exposure augments behavioral response to psychostimulants in a process termed cross-sensitization. Brain-derived neurotrophic factor (BDNF) mediates synaptic plasticity and cellular responses to stress and drugs of abuse. We previously showed that repeated social defeat stress persistently
Noradrenaline activation of neurotrophic pathways protects against neuronal amyloid toxicity.
Scott E Counts,Elliott J Mufson
Journal of Neurochemistry null
Sensitized activation of Fos and brain-derived neurotrophic factor in the medial prefrontal cortex and ventral tegmental area accompanies behavioral sensitization to amphetamine.
Fanous S, Lacagnina MJ, Nikulina EM, Hammer RP Jr.
Neuropharmacology null
Nicolás Unsain et al.
Journal of neurochemistry, 111(2), 428-440 (2009-08-19)
Brain-derived neurotrophic factor (BDNF) is involved in many aspects of neuronal biology and hippocampal physiology. Status epilepticus (SE) is a condition in which prolonged seizures lead to neuronal degeneration. SE-induced in rodents serves as a model of Temporal Lobe Epilepsy
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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