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由来生物
rabbit
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human
メーカー／製品名
Chemicon®
テクニック
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... AIFM1(9131)
特異性
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami & Kroemer 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al. 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and is widely expressed (Susin et al. 1999).
免疫原
Epitope: N-terminus
Rabbit anti-AIF (N-terminus of mature AIF) polyclonal antibody was raised against a peptide corresponding to amino acids 109 to 122 of human AIF (Susin et al. 1999).
アプリケーション
Research Category
アポトーシス及び癌
アポトーシス及び癌
Research Sub Category
アポトーシス-追加
アポトーシス-追加
This Anti-AIF Antibody, N-terminus is validated for use in WB for the detection of AIF.
Western blot: 0.25 to 1 μg/mL.
K562 cell lysate from HeLa cells can be used as a positive control and a 67 kDa band should be detected.
Optimal working dilutions must be determined by end user.
K562 cell lysate from HeLa cells can be used as a positive control and a 67 kDa band should be detected.
Optimal working dilutions must be determined by end user.
関連事項
Replaces: 04-430
物理的形状
Format: Purified
Purified IgG by ion exchange chromatography. Liquid in PBS containing 0.02% sodium azide.
保管および安定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB16502:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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