AB15500

Anti-Calcium Transporter 1 Antibody

Chemicon^®, from rabbit

• 別名: CaT-1, ECAC2
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 300
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: rat
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; western blot: suitable
• NCBIアクセッション番号: NM_018646.2
• UniProtアクセッション番号: Q9H1D0
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: rat ... Slc7A1(25648)

詳細

詳細

CaT-1 (rat/mouse 727 aa; human 725 aa, chromosome 7q33-q34; ~85 kDa) is also known as ECAC2, CAT-like (CATL) or TRPV6 (transient receptor potential cation channel, subfamily V, member 6) contains a cytoplasmic N-terminus, 6 ankyrin repeat domains, 6 transmembrane domains, an extracellular pore region between TM5-6, and cytoplasmic C-terminus. CaT-1 shows 75% sequence identity to epithelial Ca++ channel (ECaC) protein from rabbit kidney. In rat, CaT-1 transcript (3-Kb) is expressed in duodenum, proximal jejunum cecum and colon but not in kidney; whereas ECaC in rabbit is expressed in apical cell membranes in distal part of nephron (kidney), duodenum, jejunum and placenta.

特異性

Calcium transporter 1 (CaT-1, ECAC2). The immunogen sequence has no significant homology with CaT-2 or other proteins.

Other species have not yet been tested. The immunogen sequence is 100% conserved in mouse, 88% in humans and 94% in Cat-1/htrp8A and htrp8b proteins.

免疫原

Synthetic peptide near the C-terminus of rat CaT-1.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
イオンチャネル及びトランスポーター

Anti-Calcium Transporter 1 Antibody detects level of Calcium Transporter 1 & has been published & validated for use in ELISA & WB.

Western blot: 1-10 µg/mL using ECL. The expected size of CaT-1 is ~83 kDa. However, posttranslational modification may alter its size depending upon the species and cell type.
Immunohistochemistry: Not tested. It is recommended that the antibody be tried at 2-20 µg/mL
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide/well.
Optimal working dilutions must be determined by end user.

物理的形状

ImmunoAffinity Purified

Liquid in PBS with 0.1% BSA.

保管および安定性

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids