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詳細
The BacMagic-2 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. Like BacMagic DNA, BacMagic-2 DNA is derived from an AcNPV genome, contains an ORF 1629 deletion which prevents nonrecombinant virus from replicating in insect cells, and contains a deletion of the nonessential chitinase (chiA) gene, to improve production of secreted and membrane-targeted proteins. BacMagic-2 DNA have an additional deletion of the v-cath gene, a cathespsin-like cysteine protease, resulting in significantly improved quality and yield for most target proteins. The BacMagic-2 Transfection Kit contains the BacMagic-2 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and BacVector Medium, for optimal transfection performance.
Not available for sale in Japan
Not available for sale in Japan
構成
- 0.5 µg : BacMagic-2 DNA
- 50 µl : Insect GeneJuice Transfection Reagent
- 2 x 1: mlSf9 Cells
- 1 L: BacVector Insect Cell Medium
- 2 µg : Transfection Control Plasmid
警告
Toxicity: Multiple Toxicity Values, refer to MSDS
保管および安定性
Ship Code: Multiple Storage ConditionsStorage: Multiple storage requirements
法的情報
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
試験成績書（COA）
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