71138

Rosetta-gami B(DE3)pLacI Competent Cells - Novagen

Escherichia coli, rod shaped

• UNSPSCコード: 41106202
• NACRES: NA.81

特性

• product name: Rosetta-gami B(DE3)pLacI Competent Cells - Novagen, Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance the expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.
• 由来生物: Escherichia coli
• 品質水準: 100
• メーカー／製品名: Novagen^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• 成長モード: adherent or suspension
• 形態: rod shaped
• テクニック: microbiological culture: suitable
• 輸送温度: dry ice
• 保管温度: −70°C

詳細

詳細

Genotype: F^–ompT hsdS_B (r_B^– m_B^–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLacIRARE (Cam^R, Kan^R, Tet^R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance the expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.

This product has been discontinued.


Expression host strains for pETBlue<TMSYMBOL></TMSYMBOL> and pTriEx<TMSYMBOL></TMSYMBOL> vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.

Note that these strains are not recommended for use with pET, pACYCDuet<TMSYMBOL></TMSYMBOL>, pCDF, pRSF, pCOLADuet<TMSYMBOL></TMSYMBOL> or pETcoco<TMSYMBOL></TMSYMBOL> vectors.

Rosetta-gami B strains combine the key features of BL21 (and its Tuner<TMSYMBOL></TMSYMBOL> derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors. In Rosetta-gami B(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

構成

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta-gami B(DE3)pLacI Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

法的情報

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2