70608

pETBlue-1 DNA - Novagen

Novagen′s pETBlue-1 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes.

• UNSPSCコード: 41106610
• NACRES: NA.51

特性

• 品質水準: 100
• 形状: liquid
• メーカー／製品名: Novagen^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

The pETBlue-1 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-1 facilitates the expression of native unfused proteins and allows convenient subcloning of target genes already fused to existing detection and purification tags. The EcoR V cloning site is appropriately spaced downstream of an E. coli ribosome binding site. Inserts must encode an ATG start codon at their 5′ end if expression is desired. The sequence is numbered from the first base of the T7 promoter sequence.

警告

Toxicity: Standard Handling (A)

法的情報

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable