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詳細
The pET-32 Xa/LIC vector is an Xa/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag thioredoxin protein, and His•Tag and STag coding sequences that are cleavable with Factor Xa protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Xa protease cleavage site (IleGluGlyArg↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification...
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
構成
•1 µgXa/LIC Vector
•10 µlXa/LIC Control Insert
•25 UT4 DNA Polymerase, LIC-qualified
•50 µl10X T4 DNA Polymerase Buffer
•100 µl100 mM DTT
•50 µl25 mM EDTA
•40 µl25 mM dGTP
•1.5 mlNuclease-free Water
•22 × 50 µlNovaBlue GigaSingles Competent Cells
•0.2 mlBL21(DE3) Competent Cells
•0.2 mlBL21(DE3)pLysS Competent Cells
•5 × 2 mlSOC Medium
•10 µlTest Plasmid
•10 µlXa/LIC Control Insert
•25 UT4 DNA Polymerase, LIC-qualified
•50 µl10X T4 DNA Polymerase Buffer
•100 µl100 mM DTT
•50 µl25 mM EDTA
•40 µl25 mM dGTP
•1.5 mlNuclease-free Water
•22 × 50 µlNovaBlue GigaSingles Competent Cells
•0.2 mlBL21(DE3) Competent Cells
•0.2 mlBL21(DE3)pLysS Competent Cells
•5 × 2 mlSOC Medium
•10 µlTest Plasmid
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
法的情報
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Eye Irrit. 2
保管分類コード
10-13 - German Storage Class 10 to 13
WGK
WGK 3
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
70072-3:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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