650366-M

Tryptase, Human Lung

• Enzyme Commission number: 3.4.21.59 (BRENDA, IUBMB)
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• フォーム: liquid
• 品質水準: 100
• 比活性: ≥50 units/mg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• 保管温度: −20°C

詳細

詳細

Native tryptase from human lung. Tryptase is a serine protease stored in mast cell granules. A tetrameric enzyme composed of four non-covalently bound subunits. Each subunit is reported to contain one active site. Reported to upregulate neoangiogenesis in carcinogenesis of the uterine cortex. Tryptase is also an important mediator of allergic reactions that activates G-protein-coupled receptors through proteolysis, thus amplifying histamine release.

Native tryptase from human lung. Tryptase is a serine protease stored in mast cell granules. A tetrameric enzyme composed of four non-covalently bound subunits. Each subunit is reported to contain one active site. Tryptase is an important mediator of allergic reactions that activates G-protein-coupled receptors through proteolysis, thus amplifying histamine release.

警告

Toxicity: Irritant (B)

単位の定義

One unit is defined as the amount of enzyme that gives a change of 1 absorbance per min at 410 nm using N-benzoyl-Phe-Val-Arg-pNA as a substrate in 30 min at 25°C.

物理的形状

In 1 M NaCl, 50 mM NaOAc, 50 µM heparin, pH 5.0.

調製ノート

Prepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

再構成

Following initial thaw, aliquot and freeze (-20°C).

その他情報

Benitez-Bribiesca, L., et al. 2001. J. Histochem. Cytochem.49, 1061.
He, S., et al. 1998. J. Pharmacol. Exp. Ther. 286, 289.
Molino, M., et al. 1997. J. Biol. Chem. 272, 4043.
Schwartz, L.B., and Bradford, T.R. 1986. J. Biol. Chem. 261, 7372.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1