5.00501

iPSC Induction Enhancer II, OAC1

• 別名: iPSC Induction Enhancer II, OAC1, Induced Pluripotent Stem Cells Induction Enhancer II, Oct4-Activating Compound 1, N-(1H-Pyrrolo[2,3-c]pyridin-5-yl)benzamide
• 実験式（ヒル表記法）: C₁₄H₁₁N₃O
• CAS番号: 300586-90-7
• 分子量: 237.26
• UNSPSCコード: 12352200

特性

• アッセイ: ≥99% (HPLC)
• 品質水準: 100
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: off-white
• 溶解性: DMSO: 100 mg/mL
• 保管温度: 2-8°C
• InChI: 1S/C14H11N3O/c18-14(10-4-2-1-3-5-10)17-13-8-11-6-7-15-12(11)9-16-13/h1-9,15H,(H,16,17,18)
• InChI Key: HWJRIFZDXJKJJN-UHFFFAOYSA-N

詳細

詳細

A cell-permeable pyrrolopyridinyl-benzamide compound that is reported to enhance the efficiency of 4F/4TF- (OSKM; Oct44, Sox2, Klf4, c-Myc) driven iPSC generation from MEFs cultured in either feeder cell-containing ESC media (induction efficiency 20 d post viral transduction = 0.43% vs. 0.186%, respectively, with or without 1 µM OAC1 treatment from d2 to d20) or in feeder-/serum-free iSF1 media (efficiency 10 d post viral transduction = 2.75% vs. 0.68%, respectively, with or without 1 µM OAC1 treatment from d2 to d10). OCA1 is shown to increase cellular Oct4, Nanog, Sox2, and Tet1 mRNA levels via a yet unidentified mechanism, although OAC1 is reported not to affect Oct4 promoter methylation, cellular Wnt signaling, or p53 and p21 mRNA levels. Six other iPSC reprogramming improving reagents, AM580, AzaC, BIX-01294 (Cat. No. 382190), Tranylcypromine (Cat. No. 616431), VPA (Cat. No. 676380), and Vitamin C (Cat. No. 1813), in comparison, do not exhibit Oct4 and Nanog promoters enhancing activity, while BIO (Cat. No. 361550) is demonstrated to be 3.8- and 6-fold less potent than OAC1, respectively, in inducing Oct4 and Nanog transcription (in human ESCs-based reporter assays).

A cell-permeable pyrrolopyridinyl-benzamide compound that is reported to enhance the efficiency of 4F/4TF-induced iPSC generation from MEFs cultured in either feeder cell-containing ESC media or in feeder-/serum-free iSF1 media (1 µM OAC1 treatment starts 48 h after viral transduction). OCA1 is shown to increase cellular Oct4, Nanog, Sox2, and Tet1 mRNA levels via a yet unidentified mechanism, although OAC1 is reported not affect Oct4 promoter methylation, cellular Wnt signaling, or p53 and p21 mRNA levels. Six other iPSC reprogramming improving reagents, AM580, AzaC, BIX-01294 (Cat. No. 382190), Tranylcypromine (Cat. No. 616431), VPA (Cat. No. 676380), and Vitamin C (Cat. No. 1813), in comparison, do not exhibit Oct4 and Nanog promoters enhancing activity, while BIO (Cat. No. 361550) is demonstrated to be 3.8- and 6-fold less potent than OAC1, respectively, in inducing Oct4 and Nanog transcription.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

生物化学的／生理学的作用

Cell permeable: yes

Reversible: yes

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Use only fresh DMSO for reconstitution.

その他情報

Li, W., et al. 2012. Proc. Natl. Acad. Sci. USA109, 20853.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable