391334

BES

ULTROL^® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

• 別名: BES, Free Acid, ULTROL^® Grade, N,N- bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
• 実験式（ヒル表記法）: C₆H₁₅NO₅S
• CAS番号: 10191-18-1
• 分子量: 213.25
• MDL番号: MFCD00007533
• UNSPSCコード: 12161700
• NACRES: NA.25

特性

• 品質水準: 100
• アッセイ: ≥99% dry basis (titration)
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 色: white
• pKa (20 °C): 7.12
• 溶解性: water: 1 mg/mL
• 微量陽イオン: heavy metals: ≤1 ppm
• 輸送温度: ambient
• 保管温度: 15-25°C
• InChI: 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
• InChI Key: AJTVSSFTXWNIRG-UHFFFAOYSA-N

詳細

詳細

Absorbance (100 mM, 260 nm):≤0.05.

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pK_a of 7.12 at 20°C.

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.

警告

Toxicity: Standard Handling (A)

再構成

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.

その他情報

De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable