368044

Granzyme A, Human, Recombinant, E. coli

Granzyme A, Human, Recombinant, E. coli, is a trypsin-like serine protease secreted by cytotoxic T cells and natural killer cells. Induces cell death through caspase-independent mechanism.

• 別名: GzmA
• Enzyme Commission number: 3.4.21.78 (BRENDA, IUBMB)
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• リコンビナント: expressed in E. coli
• 品質水準: 100
• 形状: liquid
• 比活性: ≥100 units/μg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• 輸送温度: wet ice
• 保管温度: −70°C

詳細

詳細

Recombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease found in the cytotoxic granules and secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is a pro-apoptotic enzyme that induces cell death through a caspase-independent mechanism. Granzyme A-induced apoptosis results in single-stranded DNA nicking, nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin, activate the thrombin receptor on neuronal cells and astrocytes, cleave and activate interleukin-1β, and cleave putative HLA-associated protein II (PHAP II). Recombinant human Granzyme A expressed in E. coli.

Recombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. A proapoptotic enzyme that induces cell death through caspase-independent mechanism, causing single-stranded DNA nicking and other apoptotic features including nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin and activate the thrombin receptor on neuronal cells and astrocytes. It also cleaves and activates interleukin-1β. Note: The M.W. is that of a homodimer.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.

物理的形状

In 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.

その他情報

Fan, Z., et al. 2002. Mol. Cell. Biol.22, 2810.
Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol.20, 323.
Beresford, P.J., et al. 2001. J. Biol. Chem.276, 43285.
Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol.244, 68.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable