36-012-I

Anti-nitro-α/α-Synuclein (Tyr39) Antibody, clone nSyn14

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: nSyn14, monoclonal
• 分子量: calculated mol wt 14.46 kDa
• 精製方法: using protein G
• 交差性: human, mouse
• 包装: antibody small pack of 100 μL
• テクニック: ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: N-terminal half
• タンパク質IDアクセッション番号: NP_000336
• UniProtアクセッション番号: P37840
• 輸送温度: ambient
• ターゲットの翻訳後修飾: mutation (Tyr39)
• 遺伝子情報: human ... SCNA(6622)

詳細

詳細

Alpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP; PARK1) gene (Gene ID: 6622) in human. α-synuclein is abundantly expressed in the brain and is found in a classic amyloid fibril form within the intra-neuronal Lewy body deposits of Parkinson′s disease brains. In its monomeric form it participates in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. It acts by increasing local calcium release from microdomains, which is essential for the enhancement of ATP-induced exocytosis. In its multimeric form it acts as a molecular chaperone assisting in the folding of synaptic fusion components called SNAREs at presynaptic membrane. It is also shown to regulate dopamine release and transport. It reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Post-translationally it can be phosphorylated, predominantly on serine residues. Phosphorylation of serine 129 is reported to be selective and extensive in synucleinopathy lesions. In addition, increased phosphorylation of Tyr125, nitration of Tyr39, and glycation of α-synuclein have been reported in Parkinsons disease subjects. Nitration of tyrosine residues in α-synuclein is observed in the signature inclusions of Parkinsons disease, dementia with Lewy bodies, the Lewy body variant of Alzheimer s disease, and multiple system atrophy brains. EAAC1 -/- mice display age-dependent loss of dopaminergic neurons in the substantia nigra pars compacta and this neuronal loss is accompanied by increased nitrosylated α-synuclein and microglial activation. Genetic alterations of SNCA gene are reported to result in aberrant polymerization into fibrils, which is associated with several neurodegenerative diseases (synucleinopathies). (Ref.: Miranda, HV., et al. (2017). Sci. Rep. 7; 13713; Berman, AE., et al. (2011). Ann. Neurol. 69(3); 509 520; Giasson, BI., et al. (2000). Science. 290(5493); 985-989).

特異性

Clone nSyn14 is a mouse monoclonal antibody that detects a/b synuclein nitrated at tyrosine 39.

免疫原

Full-length recombinant human α-synuclein nitrated at tyrosine 39.

アプリケーション

Anti-nitro-α/β-Synuclein (Tyr39), clone nSyn14, Cat. No. 36-012-I, is a mouse monoclonal antibody that detects α/β-Synuclein nitrated on tyrosine 39 and is tested for use in ELISA, Immunohistochemistry, and Western Blotting.

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.

Tested Applications

ELISA Analysis: A representative lot detected nitro- α/β-Synuclein (Tyr39) in ELISA applications (Giasson, B.I., et al. (2000). Science. 290(5493):985-9).
Immunohistochemistry Analysis: A representative lot detected nitro- α/β-Synuclein (Tyr39) in Immunohistochemistry applications (Maki, R.A., et al. (2019). Free Radic Biol Med. 141:115-140; Giasson, B.I., et al. (2000). Science. 290(5493):985-9).
Western Blotting Analysis: A 1:500 dilution from a representative lot detected nitro- α/β-Synuclein in Various a-synuclein protein samples (chemically nitrated or untreated) (Courtesy of Sarah Wright, Nitrome Biosciences, Inc., Brisbane, CA USA).
ELISA Analysis: Various dilutions of this antibody detected nitro- α/β-Synuclein in various a-synuclein protein samples (chemically nitrated or untreated) (Courtesy of Sarah Wright, Nitrome Biosciences, Inc., Brisbane, CA USA). .
Western Blotting Analysis: A representative lot detected nitro- α/β-Synuclein (Tyr39) in Western Blotting applications (Miranda, H.V., et al. (2017). Sci Rep. 7(1):13713; Fernandez, E., et al. (2014). Antioxid Redox Signal. 21(15):2143-8; Hung, L.W., et al. (2012). J Exp Med. 209(4):837-54; Giasson, B.I., et al. (2000). Science. 290(5493):985-9).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

物理的形状

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable