322327

Diphtheria Toxin, CRM Mutant

Diphtheria Toxin, CRM197 Mutant, CAS 92092-36-9, is a nontoxic material with a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity.

• 別名: Diphtheria Toxin, CRM Mutant
• CAS番号: 92092-36-9
• UNSPSCコード: 12352202

特性

• 品質水準: 100
• フォーム: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• 輸送温度: ambient
• 保管温度: 2-8°C

詳細

詳細

CRM₁₉₇ is a nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity. Does not catalyze the ADP ribosylation of EF-2 factor. Immunologically, the CRM₁₉₇ protein is identical to the wild-type toxin.

Cross-reactive material of diphtheria toxin (CRM₁₉₇). A nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A that results in complete loss of enzymatic activity. Inhibits smooth muscle cell proliferation in a dose-dependent manner. Immunologically, the CRM₁₉₇ is identical to the wild-type toxin and is useful as a carrier protein of conjugate vaccines.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
Mutant with loss of enzymatic activity to catalyze the ADP ribosylation of EF-2 factor

Reversible: no

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from 10 mM sodium phosphate buffer, 5% lactose, pH 7.4.

再構成

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C.

Please refer to vial label for lot-specific reconstitution volume.

アナリシスノート

One major band by SDS-PAGE

その他情報

Kusano, I., et al. 2001. Cell Struct. Funct.26, 279.
Kaefer, M., et al. 2000. J. Urol.163, 580.
Gupta, R.K., et al. 1997. Vaccine15, 1341.
Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol.251, 175.
Giannini, G., et al. 1984. Nucleic Acids Res.12, 4063.
Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable