196810

Bax-Inhibiting Peptide, V5

A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection.

• 別名: Bax-Inhibiting Peptide, V5, BIP-V5, H-VPMLK-OH
• 実験式（ヒル表記法）: C₂₇H₅₀N₆O₆S
• 分子量: 586.79
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• フォーム: lyophilized solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic); protect from light
• 色: white
• 溶解性: water: 1 mg/mL
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection. Functions as effectively as the Caspase Inhibitor VI (Z-VAD-FMK; Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also effectively blocks caspase-independent necrotic cell death. Shown to be Ku70 competitive, interact with Bax, prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days). Negative control peptide is also available (Cat. No. 196811).

A cell-permeable pentapeptide derived from the Ku70-Bax inhibiting domain that has been shown to offer protection against apoptosis in cells of human, mouse, rant, and porcine origin. Reported to function as effectively as the caspase inhibitor, Z-VAD-FMK (Caspase Inhibitor VI, Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also reported to effectively block caspase-independent necrotic cell death. Shown to interact with Bax in a Ku70-competitive manner and prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days).

生物化学的／生理学的作用

Cell permeable: yes

Primary Target
Bax mediated apoptosis

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 50-200 µM against Bax mediated apoptosis

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

シーケンス

H-Val-Pro-Met-Leu-Lys-OH

再構成

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Storage in glass vials is not recommended as loss of activity may occur.

その他情報

Yoshida, T., et al. 2004. Biochem. Biophys. Res. Commun.321, 961.
Sawada, M., et al. 2003. Nature Cell Biol.5, 352.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable