171536

AMPK (α1/β1/γ1), His•Tag^®, Human, Recombinant, S. frugiperda

full length enzyme consisting of subunits A1, B1, and G1

• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• リコンビナント: expressed in insect cells (S. frugiperda)
• 品質水準: 100
• アッセイ: ≥95% (SDS-PAGE)
• 形状: liquid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• 輸送温度: wet ice
• 保管温度: −70°C

詳細

詳細

Full length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening.

Full length, recombinant, human AMPK subunits (A1, B1, and G1), each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The active complex is useful in the study of kinase activity regulation and inhibitor screening. M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1).

M.W. = 68,000 (A1), 38,000 (B1), 40,000 (G1)

警告

Toxicity: Standard Handling (A)

物理的形状

In 300 mM NaCl, 150 mM Imidazole, 50 mM Sodium Phosphate, 0.2 mM DTT, 0.1 mM PMSF, 25% glycerol, pH 7.0.

再構成

Following initial thaw, aliquot and freeze (-70°C).

その他情報

Scott J.W., 2007. EMBO J26, 806.
Minokoshi Y et al. 2004. Nature428, 569.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable