171263-M

AMPK Signaling Activator IX, F17

The AMPK Signaling Agonist, F17, also referenced under CAS 280112-24-5, controls the biological activity of AMPK. This small molecule/inhibitor is primarily used for Biochemicals applications.

• 別名: AMPK Signaling Activator IX, F17, 4-hydroxy-1-isobutyl-N-(5-methylthiazol-2-yl)-2-oxo-1,2-dihydroquinoline-3-carboxamide
• 実験式（ヒル表記法）: C₁₈H₁₉N₃O₃S
• CAS番号: 280112-24-5
• 分子量: 357.43
• UNSPSCコード: 12352200

特性

• アッセイ: ≥95% (HPLC)
• 形状: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: off-white
• 溶解性: DMSO: 5 mg/mL, clear, colorless
• 保管温度: 2-8°C
• InChI: 1S/C18H19N3O3S/c1-10(2)9-21-13-7-5-4-6-12(13)15(22)14(17(21)24)16(23)20-18-19-8-11(3)25-18/h4-8,10,22H,9H2,1-3H3,(H,19,20,23)
• InChI Key: QSZAFLFWFQTCJK-UHFFFAOYSA-N

詳細

詳細

A cell-permeable 4-hydroxy-2-oxo quinoline carboxamide that acts as an agonist of AMPK signaling. It is shown to reduce fat storage in C. elegans (EC₅₀ ~ 0.1 µM) and reduce the expression level of fat-7 (a C. elegans ortholog of mammalian stearoyl-CoA desaturase-1), through aak-1 (an ortholog for the AMPK-α1 catalytic subunit) and K08F8.2 (a transcription factor) dependent mechanisms. In addition, it markedly reduces the number of lipid droplets in HepG2 human hepatocarcinoma cells at 25 µM, and elicits the phosphorylation and inactivation of acetyl coenzyme A carboxylase (ACC, the enzyme that catalyzes the rate-limiting step in de novo fatty acid synthesis). F17-induced ACC phosphorylation can be abrogated by simultaneous treatment with an AMPK inhibitor compound C (Cat. No. 171260).

警告

Toxicity: Standard Handling (A)

調製ノート

Warming to 55-60°C is required for complete solubilization.

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Lemieux, G.A., et al. 2011. Nat. Chem. Biol.7, 206.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable