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物質
PEEK column
品質水準
認証
suitable for USP L1
製品種目
Chromolith®
特徴
endcapped
包装
1 ea of
標識化の程度
18% Carbon loading
パラメーター
200 bar pressure
45 °C max. temp.
テクニック
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)
カラムL × 内径
5 cm × 2 mm
マトリックス
monolithic silica
マトリックス活性基
C18 bonding phase
ポアサイズ
1.5 μm macroporosity
13 nm mesoporosity
operating pH range
2-7.5
分離法
reversed phase
保管温度
no temp limit
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詳細
Chromolith® FastGradient RP-18 endcapped 50-2 columns have macropores with 1.5 μm diameter, giving a column efficiency exceeding 100,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® FastGradient RP-18 endcapped 50-2 columns fall under the USP L1 Classification. The column efficiency is comparable to a 3-4 μm particle size fully porous particulate column. Chromolith® FastGradient RP-18 endcapped 50-2 columns are perfectly suitable for LC-MS applications.
The column dimension of 50 mm length and 2 mm I.D. is the best choice for fast separations on any U/HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enable:
- Rapid separations at very low column back-pressure
- High matrix tolerance, allowing time and cost savings with simplified workflows
- Cost-savings due to extended column lifetimes
- Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-2 guard cartridges (3 pieces) [1.52009] with the Chromolith® Guard Cartridge Holder (kit) 5 mm x 2 mm [1.52004].
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467].
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
アナリシスノート
Pressure: ≤ 30 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 100000
Capacity factor (Anthracene): 3.6 - 4.3
Tailing (USP): 0.86 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 2.30 - 2.60
法的情報
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
関連コンテンツ
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
低分子とは、通常、分子量900ダルトン未満のイオンおよび化合物を指します。これらの化合物は、さまざまな化学的性質(修飾)を有するシリカ粒子またはモノリス型の固定相を主に用いるHPLC、UHPLCおよびLC-MSを用いて、効率よく分離することが可能です。
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