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Merck

1.50445

Supelco

SeQuant ZIC®-HILIC 200Å (3.5 µm) HPLC Columns

L × I.D. 50 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

別名:

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

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About This Item

UNSPSCコード:
41115709

product name

SeQuant ZIC-HILIC 3.5µm,200Å 50 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC

物質

PEEK-lined stainless steel column

品質水準

認証

suitable for USP L114

製品種目

SeQuant

特徴

endcapped: no

パラメーター

550 bar pressure
70 °C max. temp.

テクニック

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

カラムL × 内径

5 cm × 2.1 mm

マトリックス

fully porous particle

マトリックス活性基

ZIC-HILIC bonding phase

粒径

3.5 μm

ポアサイズ

200 Å pore size

使用pH

2-7.5

分離法

hydrophilic interaction (HILIC)

保管温度

no temp limit

詳細

SeQuant ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 50 mm length x 2.1 mm ID is the optimal choice for the fast separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant ZIC®-HILIC for analysis of melamine and related substances. SeQuant HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

法的情報

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

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